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Highly sensitive CE-ESI-MS analysis of N-glycans from complex biological samples

Author

Listed:
  • Guinevere S. M. Lageveen-Kammeijer

    (Center for Proteomics and Metabolomics)

  • Noortje Haan

    (Center for Proteomics and Metabolomics)

  • Pablo Mohaupt

    (Center for Proteomics and Metabolomics)

  • Sander Wagt

    (Center for Proteomics and Metabolomics)

  • Mike Filius

    (Center for Proteomics and Metabolomics
    TU Delft, BioNanoScience Department)

  • Jan Nouta

    (Center for Proteomics and Metabolomics)

  • David Falck

    (Center for Proteomics and Metabolomics)

  • Manfred Wuhrer

    (Center for Proteomics and Metabolomics)

Abstract

The in-depth, high-sensitivity characterization of the glycome from complex biological samples, such as biofluids and tissues, is of utmost importance in basic biological research and biomarker discovery. Major challenges often arise from the vast structural diversity of glycans in combination with limited sample amounts. Here, we present a method for the highly sensitive characterization of released N-glycans by combining a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach with linkage-specific derivatization of sialic acids and uniform cationic reducing end labelling of all glycans. This method allows the analysis of glycans at the attomole level, provides information on sialic acid isomers and enables the in-depth characterization of complex samples, even when available in minute amounts.

Suggested Citation

  • Guinevere S. M. Lageveen-Kammeijer & Noortje Haan & Pablo Mohaupt & Sander Wagt & Mike Filius & Jan Nouta & David Falck & Manfred Wuhrer, 2019. "Highly sensitive CE-ESI-MS analysis of N-glycans from complex biological samples," Nature Communications, Nature, vol. 10(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-09910-7
    DOI: 10.1038/s41467-019-09910-7
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