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Self-organization of Plk4 regulates symmetry breaking in centriole duplication

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  • Shohei Yamamoto

    (The University of Tokyo
    The University of Tokyo)

  • Daiju Kitagawa

    (The University of Tokyo)

Abstract

During centriole duplication, a single daughter centriole is formed next to the mother centriole. The molecular mechanism that determines a single duplication site remains a long-standing question. Here, we show that intrinsic self-organization of Plk4 is implicated in symmetry breaking in the process of centriole duplication. We demonstrate that Plk4 has an ability to phase-separate into condensates via an intrinsically disordered linker and that the condensation properties of Plk4 are regulated by autophosphorylation. Consistently, the dissociation dynamics of centriolar Plk4 are controlled by autophosphorylation. We further found that autophosphorylated Plk4 is already distributed as a single focus around the mother centriole before the initiation of procentriole formation, and is subsequently targeted for STIL-HsSAS6 loading. Perturbation of Plk4 self-organization affects the asymmetry of centriolar Plk4 distribution and proper centriole duplication. Overall, we propose that the spatial pattern formation of Plk4 is a determinant of a single duplication site per mother centriole.

Suggested Citation

  • Shohei Yamamoto & Daiju Kitagawa, 2019. "Self-organization of Plk4 regulates symmetry breaking in centriole duplication," Nature Communications, Nature, vol. 10(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-09847-x
    DOI: 10.1038/s41467-019-09847-x
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    Cited by:

    1. Jung-Eun Park & Tae-Sung Kim & Yan Zeng & Melissa Mikolaj & Jong Ahn & Muhammad S. Alam & Christina M. Monnie & Victoria Shi & Ming Zhou & Tae-Wook Chun & Frank Maldarelli & Kedar Narayan & Jinwoo Ahn, 2024. "Centrosome amplification and aneuploidy driven by the HIV-1-induced Vpr•VprBP•Plk4 complex in CD4+ T cells," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    2. Niccolò Banterle & Adrian P. Nievergelt & Svenja Buhr & Georgios N. Hatzopoulos & Charlène Brillard & Santiago Andany & Tania Hübscher & Frieda A. Sorgenfrei & Ulrich S. Schwarz & Frauke Gräter & Geor, 2021. "Kinetic and structural roles for the surface in guiding SAS-6 self-assembly to direct centriole architecture," Nature Communications, Nature, vol. 12(1), pages 1-14, December.

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