IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v10y2019i1d10.1038_s41467-019-09807-5.html
   My bibliography  Save this article

Transient opening of trimeric prefusion RSV F proteins

Author

Listed:
  • Morgan S. A. Gilman

    (Geisel School of Medicine at Dartmouth
    University of Texas at Austin)

  • Polina Furmanova-Hollenstein

    (Janssen Infectious Diseases and Vaccines)

  • Gabriel Pascual

    (Janssen Immunosciences, World Without Disease Accelerator)

  • Angélique van ‘t Wout

    (Janssen Vaccines & Prevention B.V
    AlphaBiomics)

  • Johannes P. M. Langedijk

    (Janssen Infectious Diseases and Vaccines)

  • Jason S. McLellan

    (University of Texas at Austin)

Abstract

The respiratory syncytial virus (RSV) F glycoprotein is a class I fusion protein that mediates viral entry and is a major target of neutralizing antibodies. Structures of prefusion forms of RSV F, as well as other class I fusion proteins, have revealed compact trimeric arrangements, yet whether these trimeric forms can transiently open remains unknown. Here, we perform structural and biochemical studies on a recently isolated antibody, CR9501, and demonstrate that it enhances the opening of prefusion-stabilized RSV F trimers. The 3.3 Å crystal structure of monomeric RSV F bound to CR9501, combined with analysis of over 25 previously determined RSV F structures, reveals a breathing motion of the prefusion conformation. We also demonstrate that full-length RSV F trimers transiently open and dissociate on the cell surface. Collectively, these findings have implications for the function of class I fusion proteins, as well as antibody prophylaxis and vaccine development for RSV.

Suggested Citation

  • Morgan S. A. Gilman & Polina Furmanova-Hollenstein & Gabriel Pascual & Angélique van ‘t Wout & Johannes P. M. Langedijk & Jason S. McLellan, 2019. "Transient opening of trimeric prefusion RSV F proteins," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-09807-5
    DOI: 10.1038/s41467-019-09807-5
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-019-09807-5
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-019-09807-5?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Daníelsson, Jón & Macrae, Robert & Uthemann, Andreas, 2022. "Artificial intelligence and systemic risk," Journal of Banking & Finance, Elsevier, vol. 140(C).
    2. Bryan S. Sibert & Joseph Y. Kim & Jie E. Yang & Zunlong Ke & Christopher C. Stobart & Martin L. Moore & Elizabeth R. Wright, 2024. "Assembly of respiratory syncytial virus matrix protein lattice and its coordination with fusion glycoprotein trimers," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    3. Cristancho Triana, Gerson Jaquin, 2023. "Actitud e intención hacia el consumo responsable en los hogares de Bogotá," Revista Tendencias, Universidad de Narino, vol. 24(1), pages 130-154, January.
    4. Ugirumurera, Juliette & Severino, Joseph & Ficenec, Karen & Ge, Yanbo & Wang, Qichao & Williams, Lindy & Chae, Junghoon & Lunacek, Monte & Phillips, Caleb, 2021. "A modeling framework for designing and evaluating curbside traffic management policies at Dallas-Fort Worth International Airport," Transportation Research Part A: Policy and Practice, Elsevier, vol. 153(C), pages 130-150.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-09807-5. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.