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Mechanisms of redundancy and specificity of the Aspergillus fumigatus Crh transglycosylases

Author

Listed:
  • Wenxia Fang

    (University of Dundee
    Guangxi Academy of Sciences)

  • Ana Belén Sanz

    (IRYCIS)

  • Sergio Galan Bartual

    (University of Dundee)

  • Bin Wang

    (Guangxi Academy of Sciences)

  • Andrew T. Ferenbach

    (University of Dundee)

  • Vladimír Farkaš

    (Center for Glycomics, Slovak Academy of Sciences)

  • Ramon Hurtado-Guerrero

    (University of Zaragoza, BIFI-IQFR (CSIC)
    Av. de Ranillas)

  • Javier Arroyo

    (IRYCIS)

  • Daan M. F. Aalten

    (University of Dundee)

Abstract

Fungal cell wall synthesis is achieved by a balance of glycosyltransferase, hydrolase and transglycosylase activities. Transglycosylases strengthen the cell wall by forming a rigid network of crosslinks through mechanisms that remain to be explored. Here we study the function of the Aspergillus fumigatus family of five Crh transglycosylases. Although crh genes are dispensable for cell viability, simultaneous deletion of all genes renders cells sensitive to cell wall interfering compounds. In vitro biochemical assays and localisation studies demonstrate that this family of enzymes functions redundantly as transglycosylases for both chitin-glucan and chitin-chitin cell wall crosslinks. To understand the molecular basis of this acceptor promiscuity, we solved the crystal structure of A. fumigatus Crh5 (AfCrh5) in complex with a chitooligosaccharide at the resolution of 2.8 Å, revealing an extensive elongated binding cleft for the donor (−4 to −1) substrate and a short acceptor (+1 to +2) binding site. Together with mutagenesis, the structure suggests a “hydrolysis product assisted” molecular mechanism favouring transglycosylation over hydrolysis.

Suggested Citation

  • Wenxia Fang & Ana Belén Sanz & Sergio Galan Bartual & Bin Wang & Andrew T. Ferenbach & Vladimír Farkaš & Ramon Hurtado-Guerrero & Javier Arroyo & Daan M. F. Aalten, 2019. "Mechanisms of redundancy and specificity of the Aspergillus fumigatus Crh transglycosylases," Nature Communications, Nature, vol. 10(1), pages 1-10, December.
  • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-09674-0
    DOI: 10.1038/s41467-019-09674-0
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    Cited by:

    1. Zhijie Yang & Yijun Qiao & Emil Strøbech & Jens Preben Morth & Grit Walther & Tue Sparholt Jørgensen & Kah Yean Lum & Gundela Peschel & Miriam A. Rosenbaum & Viola Previtali & Mads Hartvig Clausen & M, 2024. "Alligamycin A, an antifungal β-lactone spiroketal macrolide from Streptomyces iranensis," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

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