Author
Listed:
- Kun Wang
(The Wenner-Gren Institute, Stockholm University)
- David Sybers
(Vrije Universiteit Brussel)
- Hassan Ramadan Maklad
(Vrije Universiteit Brussel)
- Liesbeth Lemmens
(Vrije Universiteit Brussel)
- Charlotte Lewyllie
(Vrije Universiteit Brussel
Vrije Universiteit Brussel)
- Xiaoxiao Zhou
(Biofilm Centre, ZWU, Fakultät für Chemie, Universität Duisburg-Essen)
- Frank Schult
(Biofilm Centre, ZWU, Fakultät für Chemie, Universität Duisburg-Essen)
- Christopher Bräsen
(Biofilm Centre, ZWU, Fakultät für Chemie, Universität Duisburg-Essen)
- Bettina Siebers
(Biofilm Centre, ZWU, Fakultät für Chemie, Universität Duisburg-Essen)
- Karin Valegård
(Uppsala University)
- Ann-Christin Lindås
(The Wenner-Gren Institute, Stockholm University)
- Eveline Peeters
(Vrije Universiteit Brussel)
Abstract
Fatty acid metabolism and its regulation are known to play important roles in bacteria and eukaryotes. By contrast, although certain archaea appear to metabolize fatty acids, the regulation of the underlying pathways in these organisms remains unclear. Here, we show that a TetR-family transcriptional regulator (FadRSa) is involved in regulation of fatty acid metabolism in the crenarchaeon Sulfolobus acidocaldarius. Functional and structural analyses show that FadRSa binds to DNA at semi-palindromic recognition sites in two distinct stoichiometric binding modes depending on the operator sequence. Genome-wide transcriptomic and chromatin immunoprecipitation analyses demonstrate that the protein binds to only four genomic sites, acting as a repressor of a 30-kb gene cluster comprising 23 open reading frames encoding lipases and β-oxidation enzymes. Fatty acyl-CoA molecules cause dissociation of FadRSa binding by inducing conformational changes in the protein. Our results indicate that, despite its similarity in overall structure to bacterial TetR-family FadR regulators, FadRSa displays a different acyl-CoA binding mode and a distinct regulatory mechanism.
Suggested Citation
Kun Wang & David Sybers & Hassan Ramadan Maklad & Liesbeth Lemmens & Charlotte Lewyllie & Xiaoxiao Zhou & Frank Schult & Christopher Bräsen & Bettina Siebers & Karin Valegård & Ann-Christin Lindås & E, 2019.
"A TetR-family transcription factor regulates fatty acid metabolism in the archaeal model organism Sulfolobus acidocaldarius,"
Nature Communications, Nature, vol. 10(1), pages 1-16, December.
Handle:
RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-09479-1
DOI: 10.1038/s41467-019-09479-1
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