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Microtubule end conversion mediated by motors and diffusing proteins with no intrinsic microtubule end-binding activity

Author

Listed:
  • Manas Chakraborty

    (University of Pennsylvania
    Warwick Medical School)

  • Ekaterina V. Tarasovetc

    (University of Pennsylvania)

  • Anatoly V. Zaytsev

    (University of Pennsylvania)

  • Maxim Godzi

    (University of Pennsylvania
    Russian Academy of Sciences)

  • Ana C. Figueiredo

    (Universidade do Porto
    Universidade do Porto)

  • Fazly I. Ataullakhanov

    (Russian Academy of Sciences
    Dmitry Rogachev National Research Center of Pediatric Hematology, Oncology and Immunology
    Moscow Institute of Physics and Technology)

  • Ekaterina L. Grishchuk

    (University of Pennsylvania
    Dmitry Rogachev National Research Center of Pediatric Hematology, Oncology and Immunology)

Abstract

Accurate chromosome segregation relies on microtubule end conversion, the ill-understood ability of kinetochores to transit from lateral microtubule attachment to durable association with dynamic microtubule plus-ends. The molecular requirements for this conversion and the underlying biophysical mechanisms are elusive. We reconstituted end conversion in vitro using two kinetochore components: the plus end–directed kinesin CENP-E and microtubule-binding Ndc80 complex, combined on the surface of a microbead. The primary role of CENP-E is to ensure close proximity between Ndc80 complexes and the microtubule plus-end, whereas Ndc80 complexes provide lasting microtubule association by diffusing on the microtubule wall near its tip. Together, these proteins mediate robust plus-end coupling during several rounds of microtubule dynamics, in the absence of any specialized tip-binding or regulatory proteins. Using a Brownian dynamics model, we show that end conversion is an emergent property of multimolecular ensembles of microtubule wall-binding proteins with finely tuned force-dependent motility characteristics.

Suggested Citation

  • Manas Chakraborty & Ekaterina V. Tarasovetc & Anatoly V. Zaytsev & Maxim Godzi & Ana C. Figueiredo & Fazly I. Ataullakhanov & Ekaterina L. Grishchuk, 2019. "Microtubule end conversion mediated by motors and diffusing proteins with no intrinsic microtubule end-binding activity," Nature Communications, Nature, vol. 10(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-09411-7
    DOI: 10.1038/s41467-019-09411-7
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    Cited by:

    1. Hugo Girão & Joana Macário-Monteiro & Ana C. Figueiredo & Ricardo Silva e Sousa & Elena Doria & Vladimir Demidov & Hugo Osório & Ariana Jacome & Patrick Meraldi & Ekaterina L. Grishchuk & Helder Maiat, 2024. "α-tubulin detyrosination fine-tunes kinetochore-microtubule attachments," Nature Communications, Nature, vol. 15(1), pages 1-19, December.

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