IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v10y2019i1d10.1038_s41467-019-09386-5.html
   My bibliography  Save this article

Primed histone demethylation regulates shoot regenerative competency

Author

Listed:
  • Hiroya Ishihara

    (Tokyo University of Science)

  • Kaoru Sugimoto

    (Tokyo University of Science)

  • Paul T. Tarr

    (California Institute of Technology)

  • Haruka Temman

    (Tokyo University of Science)

  • Satoshi Kadokura

    (Tokyo University of Science)

  • Yayoi Inui

    (Tokyo University of Science)

  • Takuya Sakamoto

    (Tokyo University of Science)

  • Taku Sasaki

    (RIKEN Center for Sustainable Resource Science)

  • Mitsuhiro Aida

    (Tokyo University of Science)

  • Takamasa Suzuki

    (Chubu University)

  • Soichi Inagaki

    (National Institute of Genetics
    PREST, Japan Science and Technology Agency
    The Graduate University for Advanced Studies (SOKENDAI))

  • Kengo Morohashi

    (Tokyo University of Science)

  • Motoaki Seki

    (RIKEN Center for Sustainable Resource Science
    RIKEN Cluster for Pioneering Research)

  • Tetsuji Kakutani

    (National Institute of Genetics
    The Graduate University for Advanced Studies (SOKENDAI)
    The University of Tokyo)

  • Elliot M. Meyerowitz

    (California Institute of Technology)

  • Sachihiro Matsunaga

    (Tokyo University of Science)

Abstract

Acquisition of pluripotency by somatic cells is a striking process that enables multicellular organisms to regenerate organs. This process includes silencing of genes to erase original tissue memory and priming of additional cell type specification genes, which are then poised for activation by external signal inputs. Here, through analysis of genome-wide histone modifications and gene expression profiles, we show that a gene priming mechanism involving LYSINE-SPECIFIC DEMETHYLASE 1-LIKE 3 (LDL3) specifically eliminates H3K4me2 during formation of the intermediate pluripotent cell mass known as callus derived from Arabidopsis root cells. While LDL3-mediated H3K4me2 removal does not immediately affect gene expression, it does facilitate the later activation of genes that act to form shoot progenitors when external cues lead to shoot induction. These results give insights into the role of H3K4 methylation in plants, and into the primed state that provides plant cells with high regenerative competency.

Suggested Citation

  • Hiroya Ishihara & Kaoru Sugimoto & Paul T. Tarr & Haruka Temman & Satoshi Kadokura & Yayoi Inui & Takuya Sakamoto & Taku Sasaki & Mitsuhiro Aida & Takamasa Suzuki & Soichi Inagaki & Kengo Morohashi & , 2019. "Primed histone demethylation regulates shoot regenerative competency," Nature Communications, Nature, vol. 10(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-09386-5
    DOI: 10.1038/s41467-019-09386-5
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-019-09386-5
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-019-09386-5?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Satoyo Oya & Mayumi Takahashi & Kazuya Takashima & Tetsuji Kakutani & Soichi Inagaki, 2022. "Transcription-coupled and epigenome-encoded mechanisms direct H3K4 methylation," Nature Communications, Nature, vol. 13(1), pages 1-16, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-09386-5. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.