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Impaired cellular energy metabolism in cord blood macrophages contributes to abortive response toward inflammatory threats

Author

Listed:
  • Stephan Dreschers

    (RWTH Aachen University)

  • Kim Ohl

    (RWTH Aachen University)

  • Michael Lehrke

    (RWTH Aachen University)

  • Julia Möllmann

    (RWTH Aachen University)

  • Bernd Denecke

    (RWTH Aachen University)

  • Ivan Costa

    (RWTH Aachen University)

  • Thomas Vogl

    (University of Münster)

  • Dorothee Viemann

    (Medical University of Hannover (MHH))

  • Johannes Roth

    (University of Münster)

  • Thorsten Orlikowsky

    (RWTH Aachen University)

  • Klaus Tenbrock

    (RWTH Aachen University)

Abstract

Neonatal sepsis is characterized by hyperinflammation causing enhanced morbidity and mortality compared to adults. This suggests differences in the response towards invading threats. Here we investigate activated cord blood macrophages (CBMΦ) in comparison to adult macrophages (PBMΦ), indicating incomplete interferon gamma (IFN-γ) and interleukin 10 (IL-10)-induced activation of CBMΦ. CBMΦ show reduced expression of phagocytosis receptors and cytokine expression in addition to altered energy metabolism. In particular, IFN-γ as well as IL-10-activated CBMΦ completely fail to increase glycolysis and furthermore show reduced activation of the mTOR pathway, which is important for survival in sepsis. MTOR inhibition by rapamycin equalizes cytokine production in CBMΦ and PBMΦ. Finally, incubation of PBMΦ with cord blood serum or S100A8/A9, which is highly expressed in neonates, suppresses mTOR activation, prevents glycolysis and the expression of an PBMΦ phenotype. Thus, a metabolic alteration is apparent in CBMΦ, which might be dependent on S100A8/A9 expression.

Suggested Citation

  • Stephan Dreschers & Kim Ohl & Michael Lehrke & Julia Möllmann & Bernd Denecke & Ivan Costa & Thomas Vogl & Dorothee Viemann & Johannes Roth & Thorsten Orlikowsky & Klaus Tenbrock, 2019. "Impaired cellular energy metabolism in cord blood macrophages contributes to abortive response toward inflammatory threats," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-09359-8
    DOI: 10.1038/s41467-019-09359-8
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