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Parallel analysis of tri-molecular biosynthesis with cell identity and function in single cells

Author

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  • Samuel C. Kimmey

    (Stanford University School of Medicine
    Stanford University School of Medicine)

  • Luciene Borges

    (Stanford University School of Medicine)

  • Reema Baskar

    (Stanford University School of Medicine
    Stanford University School of Medicine)

  • Sean C. Bendall

    (Stanford University School of Medicine)

Abstract

Cellular products derived from the activity of DNA, RNA, and protein synthesis collectively control cell identity and function. Yet there is little information on how these three biosynthesis activities are coordinated during transient and sparse cellular processes, such as activation and differentiation. Here, we describe Simultaneous Overview of tri-Molecule Biosynthesis (SOM3B), a molecular labeling and simultaneous detection strategy to quantify DNA, RNA, and protein synthesis in individual cells. Comprehensive interrogation of biosynthesis activities during transient cell states, such as progression through cell cycle or cellular differentiation, is achieved by partnering SOM3B with parallel quantification of select biomolecules with conjugated antibody reagents. Here, we investigate differential de novo DNA, RNA, and protein synthesis dynamics in transformed human cell lines, primary activated human immune cells, and across the healthy human hematopoietic continuum, all at a single-cell resolution.

Suggested Citation

  • Samuel C. Kimmey & Luciene Borges & Reema Baskar & Sean C. Bendall, 2019. "Parallel analysis of tri-molecular biosynthesis with cell identity and function in single cells," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
  • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-09128-7
    DOI: 10.1038/s41467-019-09128-7
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    Cited by:

    1. David G. Priest & Takeshi Ebihara & Janyerkye Tulyeu & Jonas N. Søndergaard & Shuhei Sakakibara & Fuminori Sugihara & Shunichiro Nakao & Yuki Togami & Jumpei Yoshimura & Hiroshi Ito & Shinya Onishi & , 2024. "Atypical and non-classical CD45RBlo memory B cells are the majority of circulating SARS-CoV-2 specific B cells following mRNA vaccination or COVID-19," Nature Communications, Nature, vol. 15(1), pages 1-21, December.

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