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Hemozoin-catalyzed precipitation polymerization as an assay for malaria diagnosis

Author

Listed:
  • Omar Rifaie-Graham

    (University of Fribourg)

  • Jonas Pollard

    (University of Fribourg)

  • Samuel Raccio

    (University of Fribourg)

  • Sandor Balog

    (University of Fribourg)

  • Sebastian Rusch

    (Swiss Tropical and Public Health Institute
    University of Basel)

  • María Andrea Hernández-Castañeda

    (University of Fribourg)

  • Pierre-Yves Mantel

    (University of Fribourg)

  • Hans-Peter Beck

    (Swiss Tropical and Public Health Institute
    University of Basel)

  • Nico Bruns

    (University of Fribourg)

Abstract

Methods to diagnose malaria are of paramount interest to eradicate the disease. Current methods have severe limitations, as they are either costly or not sensitive enough to detect low levels of parasitemia. Here we report an ultrasensitive, yet low-resource chemical assay for the detection and quantification of hemozoin, a biomarker of all Plasmodium species. Solubilized hemozoin catalyzes the atom transfer radical polymerization of N-isopropylacrylamide above the lower critical solution temperature of poly(N-isopropylacrylamide). The solution becomes turbid, which can be observed by naked eye and quantified by UV-visible spectroscopy. The rate of turbidity increase is proportional to the concentration of hemozoin, with a detection limit of 0.85 ng mL−1. Malaria parasites in human blood can be detected down to 10 infected red blood cells μL−1. The assay could potentially be applied as a point-of-care test. The signal-amplification of an analyte by biocatalytic precipitation polymerization represents a powerful approach in biosensing.

Suggested Citation

  • Omar Rifaie-Graham & Jonas Pollard & Samuel Raccio & Sandor Balog & Sebastian Rusch & María Andrea Hernández-Castañeda & Pierre-Yves Mantel & Hans-Peter Beck & Nico Bruns, 2019. "Hemozoin-catalyzed precipitation polymerization as an assay for malaria diagnosis," Nature Communications, Nature, vol. 10(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-09122-z
    DOI: 10.1038/s41467-019-09122-z
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    Cited by:

    1. Aayire C. Yadem & Jillian N. Armstrong & Mustafa Sarimollaoglu & Civian Kiki Massa & Jean-Michel Ndifo & Yulian A. Menyaev & Anastasie Mbe & Kacey Richards & Martina Wade & Yushun Zeng & Ruimin Chen &, 2024. "Noninvasive in vivo photoacoustic detection of malaria with Cytophone in Cameroon," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

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