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Enhanced antibiotic resistance development from fluoroquinolone persisters after a single exposure to antibiotic

Author

Listed:
  • Theresa C. Barrett

    (Princeton University
    Rutgers Robert Wood Johnson Medical School)

  • Wendy W. K. Mok

    (Princeton University
    UConn Health)

  • Allison M. Murawski

    (Princeton University
    Rutgers Robert Wood Johnson Medical School)

  • Mark P. Brynildsen

    (Princeton University
    Princeton University)

Abstract

Bacterial persisters are able to tolerate high levels of antibiotics and give rise to new populations. Persister tolerance is generally attributed to minimally active cellular processes that prevent antibiotic-induced damage, which has led to the supposition that persister offspring give rise to antibiotic-resistant mutants at comparable rates to normal cells. Using time-lapse microscopy to monitor Escherichia coli populations following ofloxacin treatment, we find that persisters filament extensively and induce impressive SOS responses before returning to a normal appearance. Further, populations derived from fluoroquinolone persisters contain significantly greater quantities of antibiotic-resistant mutants than those from untreated controls. We confirm that resistance is heritable and that the enhancement requires RecA, SOS induction, an opportunity to recover from treatment, and the involvement of error-prone DNA polymerase V (UmuDC). These findings show that fluoroquinolones damage DNA in persisters and that the ensuing SOS response accelerates the development of antibiotic resistance from these survivors.

Suggested Citation

  • Theresa C. Barrett & Wendy W. K. Mok & Allison M. Murawski & Mark P. Brynildsen, 2019. "Enhanced antibiotic resistance development from fluoroquinolone persisters after a single exposure to antibiotic," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-09058-4
    DOI: 10.1038/s41467-019-09058-4
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    Cited by:

    1. Erica J. Zheng & Ian W. Andrews & Alexandra T. Grote & Abigail L. Manson & Miguel A. Alcantar & Ashlee M. Earl & James J. Collins, 2022. "Modulating the evolutionary trajectory of tolerance using antibiotics with different metabolic dependencies," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    2. Alexandr Safatov & Irina Andreeva & Galina Buryak & Olesia Ohlopkova & Sergei Olkin & Larisa Puchkova & Irina Reznikova & Nadezda Solovyanova & Boris Belan & Mikhail Panchenko & Denis Simonenkov, 2020. "How Has the Hazard to Humans of Microorganisms Found in Atmospheric Aerosol in the South of Western Siberia Changed over 10 Years?," IJERPH, MDPI, vol. 17(5), pages 1-19, March.

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