Author
Listed:
- Shangfeng Wang
(Fudan University)
- Yong Fan
(Fudan University)
- Dandan Li
(Fudan University)
- Caixia Sun
(Fudan University)
- Zuhai Lei
(Fudan University)
- Lingfei Lu
(Fudan University)
- Ting Wang
(Fudan University)
- Fan Zhang
(Fudan University)
Abstract
The contrast and sensitivity of in vivo fluorescence imaging has been revolutionized by molecular fluorophores operating in the second near-infrared window (NIR-II; 1000-1700 nm), but an ongoing challenge is the solvatochromism-caused quenching in aqueous solution for the long-wavelength absorbing fluorophores. Herein, we develop a series of anti-quenching pentamethine cyanine fluorophores that significantly overcome the severe solvatochromism, thus affording stable absorption/emission beyond 1000 nm with up to ~ 44-fold enhanced brightness and superior photostability in aqueous solution. These advantages allow for deep optical penetration (8 mm) as well as high-contrast and highly-stable lymphatic imaging superior to clinical-approved indocyanine green. Additionally, these fluorophores exhibit pH-responsive fluorescence, allowing for noninvasive ratiometric fluorescence imaging and quantification of gastric pH in vivo. The results demonstrate reliable accuracy in tissue as deep as 4 mm, comparable to standard pH electrode method. This work unlocks the potential of anti-quenching pentamethine cyanines for NIR-II biological applications.
Suggested Citation
Shangfeng Wang & Yong Fan & Dandan Li & Caixia Sun & Zuhai Lei & Lingfei Lu & Ting Wang & Fan Zhang, 2019.
"Anti-quenching NIR-II molecular fluorophores for in vivo high-contrast imaging and pH sensing,"
Nature Communications, Nature, vol. 10(1), pages 1-11, December.
Handle:
RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-09043-x
DOI: 10.1038/s41467-019-09043-x
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