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Bioengineered bacterial vesicles as biological nano-heaters for optoacoustic imaging

Author

Listed:
  • Vipul Gujrati

    (Technische Universität München
    Institute of Biological and Medical Imaging, Helmholtz Zentrum München)

  • Jaya Prakash

    (Institute of Biological and Medical Imaging, Helmholtz Zentrum München)

  • Jaber Malekzadeh-Najafabadi

    (Technische Universität München
    Institute of Biological and Medical Imaging, Helmholtz Zentrum München)

  • Andre Stiel

    (Institute of Biological and Medical Imaging, Helmholtz Zentrum München)

  • Uwe Klemm

    (Institute of Biological and Medical Imaging, Helmholtz Zentrum München)

  • Gabriele Mettenleiter

    (Helmholtz Zentrum München)

  • Michaela Aichler

    (Helmholtz Zentrum München)

  • Axel Walch

    (Helmholtz Zentrum München)

  • Vasilis Ntziachristos

    (Technische Universität München
    Institute of Biological and Medical Imaging, Helmholtz Zentrum München)

Abstract

Advances in genetic engineering have enabled the use of bacterial outer membrane vesicles (OMVs) to deliver vaccines, drugs and immunotherapy agents, as a strategy to circumvent biocompatibility and large-scale production issues associated with synthetic nanomaterials. We investigate bioengineered OMVs for contrast enhancement in optoacoustic (photoacoustic) imaging. We produce OMVs encapsulating biopolymer-melanin (OMVMel) using a bacterial strain expressing a tyrosinase transgene. Our results show that upon near-infrared light irradiation, OMVMel generates strong optoacoustic signals appropriate for imaging applications. In addition, we show that OMVMel builds up intense heat from the absorbed laser energy and mediates photothermal effects both in vitro and in vivo. Using multispectral optoacoustic tomography, we noninvasively monitor the spatio-temporal, tumour-associated OMVMel distribution in vivo. This work points to the use of bioengineered vesicles as potent alternatives to synthetic particles more commonly employed for optoacoustic imaging, with the potential to enable both image enhancement and photothermal applications.

Suggested Citation

  • Vipul Gujrati & Jaya Prakash & Jaber Malekzadeh-Najafabadi & Andre Stiel & Uwe Klemm & Gabriele Mettenleiter & Michaela Aichler & Axel Walch & Vasilis Ntziachristos, 2019. "Bioengineered bacterial vesicles as biological nano-heaters for optoacoustic imaging," Nature Communications, Nature, vol. 10(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-09034-y
    DOI: 10.1038/s41467-019-09034-y
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    Cited by:

    1. Mingming Zhao & Xiaohui Cheng & Pingwen Shao & Yao Dong & Yongjie Wu & Lin Xiao & Zhiying Cui & Xuedi Sun & Chuancheng Gao & Jiangning Chen & Zhen Huang & Junfeng Zhang, 2024. "Bacterial protoplast-derived nanovesicles carrying CRISPR-Cas9 tools re-educate tumor-associated macrophages for enhanced cancer immunotherapy," Nature Communications, Nature, vol. 15(1), pages 1-18, December.

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