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Imaging inflammation using an activated macrophage probe with Slc18b1 as the activation-selective gating target

Author

Listed:
  • Sung-Jin Park

    (Agency for Science, Technology and Research)

  • Beomsue Kim

    (Agency for Science, Technology and Research)

  • Sejong Choi

    (Seoul National University)

  • Sivaraman Balasubramaniam

    (Agency for Science, Technology and Research)

  • Sung-Chan Lee

    (Agency for Science, Technology and Research)

  • Jung Yeol Lee

    (Pohang University of Science and Technology (POSTECH))

  • Heon Seok Kim

    (Seoul National University)

  • Jun-Young Kim

    (Agency for Science, Technology and Research)

  • Jong-Jin Kim

    (Agency for Science, Technology and Research
    Institute for Basic Science (IBS))

  • Yong-An Lee

    (Agency for Science, Technology and Research)

  • Nam-Young Kang

    (Agency for Science, Technology and Research
    Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF))

  • Jin-Soo Kim

    (Seoul National University
    Institute for Basic Science (IBS))

  • Young-Tae Chang

    (Agency for Science, Technology and Research
    Pohang University of Science and Technology (POSTECH)
    Institute for Basic Science (IBS))

Abstract

Activated macrophages have the potential to be ideal targets for imaging inflammation. However, probe selectivity over non-activated macrophages and probe delivery to target tissue have been challenging. Here, we report a small molecule probe specific for activated macrophages, called CDg16, and demonstrate its application to visualizing inflammatory atherosclerotic plaques in vivo. Through a systematic transporter screen using a CRISPR activation library, we identify the orphan transporter Slc18b1/SLC18B1 as the gating target of CDg16.

Suggested Citation

  • Sung-Jin Park & Beomsue Kim & Sejong Choi & Sivaraman Balasubramaniam & Sung-Chan Lee & Jung Yeol Lee & Heon Seok Kim & Jun-Young Kim & Jong-Jin Kim & Yong-An Lee & Nam-Young Kang & Jin-Soo Kim & Youn, 2019. "Imaging inflammation using an activated macrophage probe with Slc18b1 as the activation-selective gating target," Nature Communications, Nature, vol. 10(1), pages 1-7, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-08990-9
    DOI: 10.1038/s41467-019-08990-9
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