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Molecular architecture of a cylindrical self-assembly at human centrosomes

Author

Listed:
  • Tae-Sung Kim

    (National Institutes of Health)

  • Liang Zhang

    (National Institutes of Health)

  • Jong Ahn

    (National Institutes of Health)

  • Lingjun Meng

    (National Institutes of Health)

  • Yang Chen

    (National Institutes of Health)

  • Eunhye Lee

    (National Institutes of Health)

  • Jeong Kyu Bang

    (Korea Basic Science Institute)

  • Jung Mi Lim

    (National Institutes of Health)

  • Rodolfo Ghirlando

    (National Institutes of Health)

  • Lixin Fan

    (Frederick National Laboratory for Cancer Research)

  • Yun-Xing Wang

    (National Institutes of Health)

  • Bo Yeon Kim

    (Korea Research Institute of Bioscience and Biotechnology)

  • Jung-Eun Park

    (National Institutes of Health)

  • Kyung S. Lee

    (National Institutes of Health)

Abstract

The cell is constructed by higher-order structures and organelles through complex interactions among distinct structural constituents. The centrosome is a membraneless organelle composed of two microtubule-derived structures called centrioles and an amorphous mass of pericentriolar material. Super-resolution microscopic analyses in various organisms revealed that diverse pericentriolar material proteins are concentrically localized around a centriole in a highly organized manner. However, the molecular nature underlying these organizations remains unknown. Here we show that two human pericentriolar material scaffolds, Cep63 and Cep152, cooperatively generate a heterotetrameric α-helical bundle that functions in conjunction with its neighboring hydrophobic motifs to self-assemble into a higher-order cylindrical architecture capable of recruiting downstream components, including Plk4, a key regulator for centriole duplication. Mutations disrupting the self-assembly abrogate Plk4-mediated centriole duplication. Because pericentriolar material organization is evolutionarily conserved, this work may offer a paradigm for investigating the assembly and function of centrosomal scaffolds in various organisms.

Suggested Citation

  • Tae-Sung Kim & Liang Zhang & Jong Ahn & Lingjun Meng & Yang Chen & Eunhye Lee & Jeong Kyu Bang & Jung Mi Lim & Rodolfo Ghirlando & Lixin Fan & Yun-Xing Wang & Bo Yeon Kim & Jung-Eun Park & Kyung S. Le, 2019. "Molecular architecture of a cylindrical self-assembly at human centrosomes," Nature Communications, Nature, vol. 10(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-08838-2
    DOI: 10.1038/s41467-019-08838-2
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    Cited by:

    1. Jung-Eun Park & Tae-Sung Kim & Yan Zeng & Melissa Mikolaj & Jong Ahn & Muhammad S. Alam & Christina M. Monnie & Victoria Shi & Ming Zhou & Tae-Wook Chun & Frank Maldarelli & Kedar Narayan & Jinwoo Ahn, 2024. "Centrosome amplification and aneuploidy driven by the HIV-1-induced Vpr•VprBP•Plk4 complex in CD4+ T cells," Nature Communications, Nature, vol. 15(1), pages 1-18, December.

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