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Measuring luteinising hormone pulsatility with a robotic aptamer-enabled electrochemical reader

Author

Listed:
  • Shaolin Liang

    (The University of Hong Kong
    Imperial College London
    Imperial College London)

  • Andrew B. Kinghorn

    (The University of Hong Kong)

  • Margaritis Voliotis

    (University of Exeter)

  • Julia K. Prague

    (Imperial College London)

  • Johannes D. Veldhuis

    (Endocrine Research Unit, Mayo School of Graduate Medical Education, Mayo Clinic)

  • Krasimira Tsaneva-Atanasova

    (University of Exeter)

  • Craig A. McArdle

    (University of Bristol)

  • Raymond H. W. Li

    (The University of Hong Kong)

  • Anthony E. G. Cass

    (Imperial College London)

  • Waljit S. Dhillo

    (Imperial College London)

  • Julian A. Tanner

    (The University of Hong Kong)

Abstract

Normal reproductive functioning is critically dependent on pulsatile secretion of luteinising hormone (LH). Assessment of LH pulsatility is important for the clinical diagnosis of reproductive disorders, but current methods are hampered by frequent blood sampling coupled to expensive serial immunochemical analysis. Here, we report the development and application of a Robotic APTamer-enabled Electrochemical Reader (RAPTER) electrochemical analysis system to determine LH pulsatility. Through selective evolution of ligands by exponential enrichment (SELEX), we identify DNA aptamers that bind specifically to LH and not to related hormones. The aptamers are integrated into electrochemical aptamer-based (E-AB) sensors on a robotic platform. E-AB enables rapid, sensitive and repeatable determination of LH concentration profiles. Bayesian Spectrum Analysis is applied to determine LH pulsatility in three distinct patient cohorts. This technology has the potential to transform the clinical care of patients with reproductive disorders and could be developed to allow real-time in vivo hormone monitoring.

Suggested Citation

  • Shaolin Liang & Andrew B. Kinghorn & Margaritis Voliotis & Julia K. Prague & Johannes D. Veldhuis & Krasimira Tsaneva-Atanasova & Craig A. McArdle & Raymond H. W. Li & Anthony E. G. Cass & Waljit S. D, 2019. "Measuring luteinising hormone pulsatility with a robotic aptamer-enabled electrochemical reader," Nature Communications, Nature, vol. 10(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-08799-6
    DOI: 10.1038/s41467-019-08799-6
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