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Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems

Author

Listed:
  • Ashot Papikian

    (University of California
    University of California)

  • Wanlu Liu

    (University of California
    University of California)

  • Javier Gallego-Bartolomé

    (University of California)

  • Steven E. Jacobsen

    (University of California
    University of California)

Abstract

Understanding genomic functions requires site-specific manipulation of loci via efficient protein effector targeting systems. However, few approaches for targeted manipulation of the epigenome are available in plants. Here, we adapt the dCas9-SunTag system to engineer targeted gene activation and DNA methylation in Arabidopsis. We demonstrate that a dCas9-SunTag system utilizing the transcriptional activator VP64 drives robust and specific activation of several loci, including protein coding genes and transposable elements, in diverse chromatin contexts. In addition, we present a CRISPR-based methylation targeting system for plants, utilizing a SunTag system with the catalytic domain of the Nicotiana tabacum DRM methyltransferase, which efficiently targets DNA methylation to specific loci, including the FWA promoter, triggering a developmental phenotype, and the SUPERMAN promoter. These SunTag systems represent valuable tools for the site-specific manipulation of plant epigenomes.

Suggested Citation

  • Ashot Papikian & Wanlu Liu & Javier Gallego-Bartolomé & Steven E. Jacobsen, 2019. "Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-08736-7
    DOI: 10.1038/s41467-019-08736-7
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