IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v10y2019i1d10.1038_s41467-019-08686-0.html
   My bibliography  Save this article

ASPP proteins discriminate between PP1 catalytic subunits through their SH3 domain and the PP1 C-tail

Author

Listed:
  • M. Teresa Bertran

    (The Francis Crick Institute)

  • Stéphane Mouilleron

    (The Francis Crick Institute)

  • Yanxiang Zhou

    (The Francis Crick Institute)

  • Rakhi Bajaj

    (University of Arizona)

  • Federico Uliana

    (Institute of Molecular Systems Biology, ETH Zürich)

  • Ganesan Senthil Kumar

    (University of Arizona)

  • Audrey Drogen

    (Institute of Molecular Systems Biology, ETH Zürich)

  • Rebecca Lee

    (The Francis Crick Institute)

  • Jennifer J. Banerjee

    (The Francis Crick Institute)

  • Simon Hauri

    (Institute of Molecular Systems Biology, ETH Zürich)

  • Nicola O’Reilly

    (Peptide Chemistry Laboratory, The Francis Crick Institute)

  • Matthias Gstaiger

    (Institute of Molecular Systems Biology, ETH Zürich)

  • Rebecca Page

    (University of Arizona)

  • Wolfgang Peti

    (University of Arizona)

  • Nicolas Tapon

    (The Francis Crick Institute)

Abstract

Serine/threonine phosphatases such as PP1 lack substrate specificity and associate with a large array of targeting subunits to achieve the requisite selectivity. The tumour suppressor ASPP (apoptosis-stimulating protein of p53) proteins associate with PP1 catalytic subunits and are implicated in multiple functions from transcriptional regulation to cell junction remodelling. Here we show that Drosophila ASPP is part of a multiprotein PP1 complex and that PP1 association is necessary for several in vivo functions of Drosophila ASPP. We solve the crystal structure of the human ASPP2/PP1 complex and show that ASPP2 recruits PP1 using both its canonical RVxF motif, which binds the PP1 catalytic domain, and its SH3 domain, which engages the PP1 C-terminal tail. The ASPP2 SH3 domain can discriminate between PP1 isoforms using an acidic specificity pocket in the n-Src domain, providing an exquisite mechanism where multiple motifs are used combinatorially to tune binding affinity to PP1.

Suggested Citation

  • M. Teresa Bertran & Stéphane Mouilleron & Yanxiang Zhou & Rakhi Bajaj & Federico Uliana & Ganesan Senthil Kumar & Audrey Drogen & Rebecca Lee & Jennifer J. Banerjee & Simon Hauri & Nicola O’Reilly & M, 2019. "ASPP proteins discriminate between PP1 catalytic subunits through their SH3 domain and the PP1 C-tail," Nature Communications, Nature, vol. 10(1), pages 1-19, December.
  • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-08686-0
    DOI: 10.1038/s41467-019-08686-0
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-019-08686-0
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/s41467-019-08686-0?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Gautam Srivastava & Meng S. Choy & Nicolas Bolik-Coulon & Rebecca Page & Wolfgang Peti, 2023. "Inhibitor-3 inhibits Protein Phosphatase 1 via a metal binding dynamic protein–protein interaction," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    2. Sarah Lemaire & Mónica Ferreira & Zander Claes & Rita Derua & Madryn Lake & Gerd Hoeven & Fabienne Withof & Xinyu Cao & Elora C. Greiner & Arminja N. Kettenbach & Aleyde Eynde & Mathieu Bollen, 2024. "PPP1R2 stimulates protein phosphatase-1 through stabilisation of dynamic subunit interactions," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    3. Christophe Royer & Elizabeth Sandham & Elizabeth Slee & Falk Schneider & Christoffer B. Lagerholm & Jonathan Godwin & Nisha Veits & Holly Hathrell & Felix Zhou & Karolis Leonavicius & Jemma Garratt & , 2022. "ASPP2 maintains the integrity of mechanically stressed pseudostratified epithelia during morphogenesis," Nature Communications, Nature, vol. 13(1), pages 1-19, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-08686-0. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.