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Differential organization of tonic and chronic B cell antigen receptors in the plasma membrane

Author

Listed:
  • Maria Angela Gomes de Castro

    (University Medical Center Göttingen)

  • Hanna Wildhagen

    (University Medical Center Göttingen)

  • Shama Sograte-Idrissi

    (University Medical Center Göttingen
    University of Göttingen Medical Center)

  • Christoffer Hitzing

    (University Medical Center Göttingen)

  • Mascha Binder

    (University Medical Center Hamburg-Eppendorf)

  • Martin Trepel

    (University Medical Center Hamburg-Eppendorf
    Augsburg Medical Center)

  • Niklas Engels

    (University Medical Center Göttingen)

  • Felipe Opazo

    (University Medical Center Göttingen
    University of Göttingen Medical Center)

Abstract

Stimulation of the B cell antigen receptor (BCR) triggers signaling pathways that promote the differentiation of B cells into plasma cells. Despite the pivotal function of BCR in B cell activation, the organization of the BCR on the surface of resting and antigen-activated B cells remains unclear. Here we show, using STED super-resolution microscopy, that IgM-containing BCRs exist predominantly as monomers and dimers in the plasma membrane of resting B cells, but form higher oligomeric clusters upon stimulation. By contrast, a chronic lymphocytic leukemia-derived BCR forms dimers and oligomers in the absence of a stimulus, but a single amino acid exchange reverts its organization to monomers in unstimulated B cells. Our super-resolution microscopy approach for quantitatively analyzing cell surface proteins may thus help reveal the nanoscale organization of immunoreceptors in various cell types.

Suggested Citation

  • Maria Angela Gomes de Castro & Hanna Wildhagen & Shama Sograte-Idrissi & Christoffer Hitzing & Mascha Binder & Martin Trepel & Niklas Engels & Felipe Opazo, 2019. "Differential organization of tonic and chronic B cell antigen receptors in the plasma membrane," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-08677-1
    DOI: 10.1038/s41467-019-08677-1
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    Cited by:

    1. Sam Daly & João Ferreira Fernandes & Ezra Bruggeman & Anoushka Handa & Ruby Peters & Sarah Benaissa & Boya Zhang & Joseph S. Beckwith & Edward W. Sanders & Ruth R. Sims & David Klenerman & Simon J. Da, 2024. "High-density volumetric super-resolution microscopy," Nature Communications, Nature, vol. 15(1), pages 1-10, December.
    2. Florian Märkl & Christoph Schultheiß & Murtaza Ali & Shih-Shih Chen & Marina Zintchenko & Lukas Egli & Juliane Mietz & Obinna Chijioke & Lisa Paschold & Sebastijan Spajic & Anne Holtermann & Janina Dö, 2024. "Mutation-specific CAR T cells as precision therapy for IGLV3-21R110 expressing high-risk chronic lymphocytic leukemia," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    3. Alexey Ferapontov & Marjan Omer & Isabelle Baudrexel & Jesper Sejrup Nielsen & Daniel Miotto Dupont & Kristian Juul-Madsen & Philipp Steen & Alexandra S. Eklund & Steffen Thiel & Thomas Vorup-Jensen &, 2023. "Antigen footprint governs activation of the B cell receptor," Nature Communications, Nature, vol. 14(1), pages 1-20, December.

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