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Structural basis of ECF-σ-factor-dependent transcription initiation

Author

Listed:
  • Wei Lin

    (Rutgers University
    Nanjing University of Chinese Medicine)

  • Sukhendu Mandal

    (Rutgers University)

  • David Degen

    (Rutgers University)

  • Min Sung Cho

    (Rutgers University)

  • Yu Feng

    (Rutgers University)

  • Kalyan Das

    (KU Leuven)

  • Richard H. Ebright

    (Rutgers University)

Abstract

Extracytoplasmic (ECF) σ factors, the largest class of alternative σ factors, are related to primary σ factors, but have simpler structures, comprising only two of six conserved functional modules in primary σ factors: region 2 (σR2) and region 4 (σR4). Here, we report crystal structures of transcription initiation complexes containing Mycobacterium tuberculosis RNA polymerase (RNAP), M. tuberculosis ECF σ factor σL, and promoter DNA. The structures show that σR2 and σR4 of the ECF σ factor occupy the same sites on RNAP as in primary σ factors, show that the connector between σR2 and σR4 of the ECF σ factor–although shorter and unrelated in sequence–follows the same path through RNAP as in primary σ factors, and show that the ECF σ factor uses the same strategy to bind and unwind promoter DNA as primary σ factors. The results define protein-protein and protein-DNA interactions involved in ECF-σ-factor-dependent transcription initiation.

Suggested Citation

  • Wei Lin & Sukhendu Mandal & David Degen & Min Sung Cho & Yu Feng & Kalyan Das & Richard H. Ebright, 2019. "Structural basis of ECF-σ-factor-dependent transcription initiation," Nature Communications, Nature, vol. 10(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-08443-3
    DOI: 10.1038/s41467-019-08443-3
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    Cited by:

    1. Jie Li & Haonan Zhang & Dongyu Li & Ya-Jun Liu & Edward A. Bayer & Qiu Cui & Yingang Feng & Ping Zhu, 2023. "Structure of the transcription open complex of distinct σI factors," Nature Communications, Nature, vol. 14(1), pages 1-12, December.

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