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High-throughput single-cell rheology in complex samples by dynamic real-time deformability cytometry

Author

Listed:
  • Bob Fregin

    (Universität Greifswald)

  • Fabian Czerwinski

    (Universität Greifswald)

  • Doreen Biedenweg

    (Universitätsmedizin Greifswald)

  • Salvatore Girardo

    (Technische Universität Dresden)

  • Stefan Gross

    (Universitätsmedizin Greifswald
    Universitätsmedizin Greifswald)

  • Konstanze Aurich

    (Universitätsmedizin Greifswald)

  • Oliver Otto

    (Universität Greifswald
    Universitätsmedizin Greifswald)

Abstract

In life sciences, the material properties of suspended cells have attained significance close to that of fluorescent markers but with the advantage of label-free and unbiased sample characterization. Until recently, cell rheological measurements were either limited by acquisition throughput, excessive post processing, or low-throughput real-time analysis. Real-time deformability cytometry expanded the application of mechanical cell assays to fast on-the-fly phenotyping of large sample sizes, but has been restricted to single material parameters as the Young’s modulus. Here, we introduce dynamic real-time deformability cytometry for comprehensive cell rheological measurements at up to 100 cells per second. Utilizing Fourier decomposition, our microfluidic method is able to disentangle cell response to complex hydrodynamic stress distributions and to determine viscoelastic parameters independent of cell shape. We demonstrate the application of our technology for peripheral blood cells in whole blood samples including the discrimination of B- and CD4+ T-lymphocytes by cell rheological properties.

Suggested Citation

  • Bob Fregin & Fabian Czerwinski & Doreen Biedenweg & Salvatore Girardo & Stefan Gross & Konstanze Aurich & Oliver Otto, 2019. "High-throughput single-cell rheology in complex samples by dynamic real-time deformability cytometry," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-08370-3
    DOI: 10.1038/s41467-019-08370-3
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    Cited by:

    1. Liang Shen & Zhenhua Tian & Kaichun Yang & Joseph Rich & Jianping Xia & Neil Upreti & Jinxin Zhang & Chuyi Chen & Nanjing Hao & Zhichao Pei & Tony Jun Huang, 2024. "Joint subarray acoustic tweezers enable controllable cell translation, rotation, and deformation," Nature Communications, Nature, vol. 15(1), pages 1-11, December.

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