IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v10y2019i1d10.1038_s41467-018-08217-3.html
   My bibliography  Save this article

Intensiometric biosensors visualize the activity of multiple small GTPases in vivo

Author

Listed:
  • Jihoon Kim

    (Korea Advanced Institute of Science and Technology (KAIST))

  • Sangkyu Lee

    (Center for Cognition and Sociality, Institute for Basic Science (IBS))

  • Kanghoon Jung

    (Max Planck Florida Institute for Neuroscience (MPFI))

  • Won Chan Oh

    (Max Planck Florida Institute for Neuroscience (MPFI)
    University of Colorado School of Medicine)

  • Nury Kim

    (Center for Cognition and Sociality, Institute for Basic Science (IBS))

  • Seungkyu Son

    (Korea Advanced Institute of Science and Technology (KAIST))

  • YoungJu Jo

    (Korea Advanced Institute of Science and Technology (KAIST))

  • Hyung-Bae Kwon

    (Max Planck Florida Institute for Neuroscience (MPFI)
    Max Planck Institute of Neurobiology)

  • Won Do Heo

    (Korea Advanced Institute of Science and Technology (KAIST)
    Center for Cognition and Sociality, Institute for Basic Science (IBS)
    Korea Advanced Institute of Science and Technology (KAIST))

Abstract

Ras and Rho small GTPases are critical for numerous cellular processes including cell division, migration, and intercellular communication. Despite extensive efforts to visualize the spatiotemporal activity of these proteins, achieving the sensitivity and dynamic range necessary for in vivo application has been challenging. Here, we present highly sensitive intensiometric small GTPase biosensors visualizing the activity of multiple small GTPases in single cells in vivo. Red-shifted sensors combined with blue light-controllable optogenetic modules achieved simultaneous monitoring and manipulation of protein activities in a highly spatiotemporal manner. Our biosensors revealed spatial dynamics of Cdc42 and Ras activities upon structural plasticity of single dendritic spines, as well as a broad range of subcellular Ras activities in the brains of freely behaving mice. Thus, these intensiometric small GTPase sensors enable the spatiotemporal dissection of complex protein signaling networks in live animals.

Suggested Citation

  • Jihoon Kim & Sangkyu Lee & Kanghoon Jung & Won Chan Oh & Nury Kim & Seungkyu Son & YoungJu Jo & Hyung-Bae Kwon & Won Do Heo, 2019. "Intensiometric biosensors visualize the activity of multiple small GTPases in vivo," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-018-08217-3
    DOI: 10.1038/s41467-018-08217-3
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-018-08217-3
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-018-08217-3?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Natalia V. Barykina & Erin M. Carey & Olena S. Oliinyk & Axel Nimmerjahn & Vladislav V. Verkhusha, 2024. "Destabilized near-infrared fluorescent nanobodies enable background-free targeting of GFP-based biosensors for imaging and manipulation," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-018-08217-3. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.