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Functional imaging of visual cortical layers and subplate in awake mice with optimized three-photon microscopy

Author

Listed:
  • Murat Yildirim

    (Massachusetts Institute of Technology
    Massachusetts Institute of Technology)

  • Hiroki Sugihara

    (Massachusetts Institute of Technology)

  • Peter T. C. So

    (Massachusetts Institute of Technology
    Massachusetts Institute of Technology)

  • Mriganka Sur

    (Massachusetts Institute of Technology
    Massachusetts Institute of Technology)

Abstract

Two-photon microscopy is used to image neuronal activity, but has severe limitations for studying deeper cortical layers. Here, we developed a custom three-photon microscope optimized to image a vertical column of the cerebral cortex > 1 mm in depth in awake mice with low (

Suggested Citation

  • Murat Yildirim & Hiroki Sugihara & Peter T. C. So & Mriganka Sur, 2019. "Functional imaging of visual cortical layers and subplate in awake mice with optimized three-photon microscopy," Nature Communications, Nature, vol. 10(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-018-08179-6
    DOI: 10.1038/s41467-018-08179-6
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    Cited by:

    1. Tong Qiu & Honghao Cao & Kunzan Liu & Li-Yu Yu & Manuel Levy & Eva Lendaro & Fan Wang & Sixian You, 2024. "Spectral-temporal-spatial customization via modulating multimodal nonlinear pulse propagation," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    2. Miroslav Stibůrek & Petra Ondráčková & Tereza Tučková & Sergey Turtaev & Martin Šiler & Tomáš Pikálek & Petr Jákl & André Gomes & Jana Krejčí & Petra Kolbábková & Hana Uhlířová & Tomáš Čižmár, 2023. "110 μm thin endo-microscope for deep-brain in vivo observations of neuronal connectivity, activity and blood flow dynamics," Nature Communications, Nature, vol. 14(1), pages 1-9, December.

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