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DNA methylation in mice is influenced by genetics as well as sex and life experience

Author

Listed:
  • Sara A. Grimm

    (Division of Intramural Research, NIEHS)

  • Takashi Shimbo

    (Division of Intramural Research, NIEHS)

  • Motoki Takaku

    (Division of Intramural Research, NIEHS)

  • James W. Thomas

    (National Human Genome Research Institute)

  • Scott Auerbach

    (National Toxicology Program, NIEHS)

  • Brian D. Bennett

    (Division of Intramural Research, NIEHS)

  • John R. Bucher

    (National Toxicology Program, NIEHS)

  • Adam B. Burkholder

    (Division of Intramural Research, NIEHS)

  • Frank Day

    (Division of Intramural Research, NIEHS)

  • Ying Du

    (Division of Intramural Research, NIEHS)

  • Christopher G. Duncan

    (Division of Intramural Research, NIEHS)

  • John E. French

    (National Toxicology Program, NIEHS)

  • Julie F. Foley

    (National Toxicology Program, NIEHS)

  • Jianying Li

    (Division of Intramural Research, NIEHS)

  • B. Alex Merrick

    (National Toxicology Program, NIEHS)

  • Raymond R. Tice

    (National Toxicology Program, NIEHS)

  • Tianyuan Wang

    (Division of Intramural Research, NIEHS)

  • Xiaojiang Xu

    (Division of Intramural Research, NIEHS)

  • Pierre R. Bushel

    (Division of Intramural Research, NIEHS)

  • David C. Fargo

    (Division of Intramural Research, NIEHS)

  • James C. Mullikin

    (National Human Genome Research Institute)

  • Paul A. Wade

    (Division of Intramural Research, NIEHS)

Abstract

DNA methylation is an essential epigenetic process in mammals, intimately involved in gene regulation. Here we address the extent to which genetics, sex, and pregnancy influence genomic DNA methylation by intercrossing 2 inbred mouse strains, C57BL/6N and C3H/HeN, and analyzing DNA methylation in parents and offspring using whole-genome bisulfite sequencing. Differential methylation across genotype is detected at thousands of loci and is preserved on parental alleles in offspring. In comparison of autosomal DNA methylation patterns across sex, hundreds of differentially methylated regions are detected. Comparison of animals with different histories of pregnancy within our study reveals a CpG methylation pattern that is restricted to female animals that had borne offspring. Collectively, our results demonstrate the stability of CpG methylation across generations, clarify the interplay of epigenetics with genetics and sex, and suggest that CpG methylation may serve as an epigenetic record of life events in somatic tissues at loci whose expression is linked to the relevant biology.

Suggested Citation

  • Sara A. Grimm & Takashi Shimbo & Motoki Takaku & James W. Thomas & Scott Auerbach & Brian D. Bennett & John R. Bucher & Adam B. Burkholder & Frank Day & Ying Du & Christopher G. Duncan & John E. Frenc, 2019. "DNA methylation in mice is influenced by genetics as well as sex and life experience," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-018-08067-z
    DOI: 10.1038/s41467-018-08067-z
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