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Munc18 and Munc13 serve as a functional template to orchestrate neuronal SNARE complex assembly

Author

Listed:
  • Shen Wang

    (Huazhong University of Science and Technology)

  • Yun Li

    (Huazhong University of Science and Technology)

  • Jihong Gong

    (Huazhong University of Science and Technology)

  • Sheng Ye

    (Chinese Academy of Sciences)

  • Xiaofei Yang

    (South-Central University for Nationalities)

  • Rongguang Zhang

    (Chinese Academy of Sciences
    Chinese Academy of Sciences)

  • Cong Ma

    (Huazhong University of Science and Technology)

Abstract

The transition of the Munc18-1/syntaxin-1 complex to the SNARE complex, a key step involved in exocytosis, is regulated by Munc13-1, SNAP-25 and synaptobrevin-2, but the underlying mechanism remains elusive. Here, we identify an interaction between Munc13-1 and the membrane-proximal linker region of synaptobrevin-2, and reveal its essential role in transition and exocytosis. Upon this interaction, Munc13-1 not only recruits synaptobrevin-2-embedded vesicles to the target membrane but also renders the synaptobrevin-2 SNARE motif more accessible to the Munc18-1/syntaxin-1 complex. Afterward, the entry of SNAP-25 leads to a half-zippered SNARE assembly, which eventually dissociates the Munc18-1/syntaxin-1 complex to complete SNARE complex formation. Our data suggest that Munc18-1 and Munc13-1 together serve as a functional template to orchestrate SNARE complex assembly.

Suggested Citation

  • Shen Wang & Yun Li & Jihong Gong & Sheng Ye & Xiaofei Yang & Rongguang Zhang & Cong Ma, 2019. "Munc18 and Munc13 serve as a functional template to orchestrate neuronal SNARE complex assembly," Nature Communications, Nature, vol. 10(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-018-08028-6
    DOI: 10.1038/s41467-018-08028-6
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    Cited by:

    1. Marieke Meijer & Miriam Öttl & Jie Yang & Aygul Subkhangulova & Avinash Kumar & Zicheng Feng & Torben W. Voorst & Alexander J. Groffen & Jan R. T. Weering & Yongli Zhang & Matthijs Verhage, 2024. "Tomosyns attenuate SNARE assembly and synaptic depression by binding to VAMP2-containing template complexes," Nature Communications, Nature, vol. 15(1), pages 1-20, December.
    2. Bhavya R. Bhaskar & Laxmi Yadav & Malavika Sriram & Kinjal Sanghrajka & Mayank Gupta & Boby K. V & Rohith K. Nellikka & Debasis Das, 2024. "Differential SNARE chaperoning by Munc13-1 and Munc18-1 dictates fusion pore fate at the release site," Nature Communications, Nature, vol. 15(1), pages 1-18, December.

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