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Chemical-Free and Reusable Cellular Analysis: Electrochemical Impedance Spectroscopy with a Transparent ITO Culture Chip

Author

Listed:
  • Sheng-Yi Hsiao

    (Instrument Technology Research Center, National Applied Research Laboratories, Taiwan)

  • Dai-Chian Chen

    (National Yang-Ming University, Taiwan)

  • Chih-Hui Yang

    (I-Shou University, Taiwan)

  • Haw-Ming Huang

    (Taipei Medical University, Taiwan)

  • Yen-Pei Lu

    (Instrument Technology Research Center, National Applied Research Laboratories, Taiwan)

  • Hui-Shun Huang

    (Instrument Technology Research Center, National Applied Research Laboratories, Taiwan)

  • Chun-Yen Lin

    (Taipei Medical University, Taiwan)

  • Yung-Sheng Lin

    (Hungkuang University, Taiwan)

Abstract

To advance innovative green technology in studying cytology, this study developed an electrochemical impedance spectroscopic (EIS) system with an indium tin oxide (ITO) culture chip module. This paper also demonstrates typical examples of solution effects and B16-F10 cell culture. Results indicate that higher concentrations of saline or albumin had lower impedance. From impedance data, cell proliferation and decline could be elucidated. The impedance soon decreased when Triton X-100 was applied to kill cells. Furthermore, the implemented transparent ITO culture chip module is experiment-friendly to perform optical inspections. The proposed green EIS system which is advantage of chemical-free and reusability can be widely applied to cytology studies in the future.

Suggested Citation

  • Sheng-Yi Hsiao & Dai-Chian Chen & Chih-Hui Yang & Haw-Ming Huang & Yen-Pei Lu & Hui-Shun Huang & Chun-Yen Lin & Yung-Sheng Lin, 2012. "Chemical-Free and Reusable Cellular Analysis: Electrochemical Impedance Spectroscopy with a Transparent ITO Culture Chip," International Journal of Technology and Human Interaction (IJTHI), IGI Global, vol. 8(3), pages 1-9, July.
    • Handle: RePEc:igg:jthi00:v:8:y:2012:i:3:p:1-9
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