IDEAS home Printed from https://ideas.repec.org/a/ibn/jmbrjl/v10y2020i1p73.html
   My bibliography  Save this article

Study of Anticancer Activity of Pratensein and Pratensein Glycoside Isolated from Cuscuta kotchiana

Author

Listed:
  • Mandana Behbahani
  • Mohaddeseh Moghaddam

Abstract

In the present paper, we demonstrate that extract of Cuscuta kotchiana is able to inhibit in vitro proliferation of two human breast cancer cell lines, MCF-7 and MDA-MB-231. The expression levels of p53, bcl-2, caspase-3 and bax genes at the mRNA and protein levels were evaluated using quantitative Real Time PCR and western blot analysis. The most active fractions of C. kotchiana were detected by NMR as pratensein and pratensein glycoside. The cytotoxic activity of pratensein glycoside was significantly more than pratensein. The expression level of bcl2 gene was decreased in cancer cells treated by both compounds at CC50 concentrations. But the expression levels of caspase-3, p53 and bax genes were increased in treated cancer cells. In conclusion, all the data demonstrated that the glycoside form of pratensein is important agent in inducing apoptosis in human breast cancer cells.

Suggested Citation

  • Mandana Behbahani & Mohaddeseh Moghaddam, 2020. "Study of Anticancer Activity of Pratensein and Pratensein Glycoside Isolated from Cuscuta kotchiana," Journal of Molecular Biology Research, Canadian Center of Science and Education, vol. 10(1), pages 1-73, December.
    • Handle: RePEc:ibn:jmbrjl:v:10:y:2020:i:1:p:73
    as

    Download full text from publisher

    File URL: https://ccsenet.org/journal/index.php/jmbr/article/download/0/0/43135/45143
    Download Restriction: no
    File URL: https://ccsenet.org/journal/index.php/jmbr/article/view/0/43135
    Download Restriction: no
    ---><---

    More about this item

    JEL classification:

    • R00 - Urban, Rural, Regional, Real Estate, and Transportation Economics - - General - - - General
    • Z0 - Other Special Topics - - General

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:ibn:jmbrjl:v:10:y:2020:i:1:p:73. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Canadian Center of Science and Education (email available below). General contact details of provider: https://edirc.repec.org/data/cepflch.html .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.