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Synthesis of DEAE-Soybean Starch Microspheres for Adhere Animal Cell Culture

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Listed:
  • Lei Zhang
  • Mingsheng Li
  • Zhongren Ma
  • Yuping Feng

Abstract

The present study outlines the synthesis of a new microcarrier for anchorage-dependent animal cell cultures. The new microcarriers were synthesized from the cross-linking soybean starch microspheres followed by modification with 2-diethylaminoethyl (DEAE). Furthermore, 5 g/100 mL of wet microspheres DEAE-soybean starch microspheres were applied in the adhere cell culture, with an inoculation density 2.0 × 105 cells/mL of BHK-21, Marc-145, and MDCK cells. The cells were shown to grow well in the DEAE-soybean starch microcarrier, with BHK-21 cells showing a higher cell density after 144 h (2.5 × 106 cells/mL) compared to cells grown on the commercial product Cytodex 1 (2.2 × 106 cells/mL). These starch microcarriers have a potential application in anchorage-dependent animal cells culture, due to its low cost and its simple process.

Suggested Citation

  • Lei Zhang & Mingsheng Li & Zhongren Ma & Yuping Feng, 2017. "Synthesis of DEAE-Soybean Starch Microspheres for Adhere Animal Cell Culture," Journal of Agricultural Science, Canadian Center of Science and Education, vol. 9(8), pages 1-91, July.
  • Handle: RePEc:ibn:jasjnl:v:9:y:2017:i:8:p:91
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    JEL classification:

    • R00 - Urban, Rural, Regional, Real Estate, and Transportation Economics - - General - - - General
    • Z0 - Other Special Topics - - General

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