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Evaluation of the Composition of Bioactive Compounds and Antioxidant Activity in Fourteen Apricot Varieties of North India

Author

Listed:
  • Sajad Wani
  • Peerzada Hussain
  • Farooq Masoodi
  • Mukhtar Ahmad
  • Touseef Wani
  • Adil Gani
  • Sarver Rather
  • Prashant Suradkar

Abstract

Variation in the content of bioactive compounds and antioxidant activity was evaluated in fourteen apricot varieties grown in north India. In the present study, the bioactive composition of apricot varieties of north Indian Kashmir was assessed by HPLC-MS/MS and their contribution towards free radical scavenging was assessed using DPPH, FRAP and ABTS assays. Studies revealed that content of bioactive compounds in apricot varieties was found to vary significantly (p ≤ 0.05) among cultivars. Content of total phenols was significantly (p ≤ 0.05) higher in Rakausilk variety (72.5±3.12 mg/100 g GAE) followed by Viva-gold (71.2±2.11 mg/100 g GAE) and Rakhchekarpo (69.6±1.65 mg/100 g GAE). Halman apricots exhibited the highest content of total carotenoids (12.2±2.13 mg/100 g) followed by Nugget (10.5±1.11 mg/100 g). HPLC analysis of apricot samples revealed the presence of fifteen phenolic acids in Halman, Venatchaa, Rakausilk and New Castle varieties; nine phenolic acid in Rakchekarpo and Sterling apricots; twelve phenolic acids in Shakanda, Nugget, Shakarpara and CITH-1 varieties; fourteen phenolic acid in Viva gold and Turkey varieties; seven phenolic acid in Khante and nineteen phenolic acids in CITH-2 apricots. Strong correlation existed between total phenolics and antioxidant activity for DPPH assay (r = 0.91) and ABTS radical inhibition (r = 0.86) while as moderate correlation existed for FRAP (r = 0.76). For DPPH and FRAP assays; EC50 values among apricot varieties were significantly (p ≤ 0.05) lower in Rakausilk (69.2, 123.6 mg/ml) followed by Viva Gold (74.3, 129.5 mg/ml) and Rakhchekarpo (77.4, 132.4 mg/ml). For ABTS.+ inhibition the EC50 values among apricot varieties were in the order of EC50(Rakausilk) < EC50(Khante) < EC50(Viva Gold). The present study reported the first time presence of p-coumaric acid-O-hexoside, isorhamnetin-O-glucouronide, Kaempferol-3-O-acetylhexoside, quercetin-3-O-rhamnoside, sinapic acid-O- hexoside, apigenin-7-O-glucoside, vitexin and luteolin-7-O-rutinoside compounds in apricot varieties of north India.

Suggested Citation

  • Sajad Wani & Peerzada Hussain & Farooq Masoodi & Mukhtar Ahmad & Touseef Wani & Adil Gani & Sarver Rather & Prashant Suradkar, 2017. "Evaluation of the Composition of Bioactive Compounds and Antioxidant Activity in Fourteen Apricot Varieties of North India," Journal of Agricultural Science, Canadian Center of Science and Education, vol. 9(5), pages 1-66, April.
    • Handle: RePEc:ibn:jasjnl:v:9:y:2017:i:5:p:66
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    JEL classification:

    • R00 - Urban, Rural, Regional, Real Estate, and Transportation Economics - - General - - - General
    • Z0 - Other Special Topics - - General

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