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The Impact of Diet on Expression of Genes Involved in Innate Immunity in Goat Blood

Author

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  • Mulumebet Worku
  • Ahmed Abdalla
  • Sarah Adjei-Fremah
  • Hamid Ismail

Abstract

Sericea Lespedeza (SL), is a high-quality, low input forage that suppresses gastro-intestinal parasites in goats. The effect of dietary SL on the expression of genes involved in innate immunity in goats has not been established. The objective of this study was to evaluate the impact of a diet containing SL on the expression of genes involved in innate immunity in goat blood. Blood was collected by jugular venipuncture from goats fed a diet of 75% SL (n = 9) and a control group (n = 7), fed a SL free diet. Blood was used to evaluate expression of (CD-14, TLR-2, TLR-4, IL-10, IL-8, IL-2, INF-r, and TNF-a). Serum was extracted and used for evaluation of the secretion of pro-inflammatory cytokines (TNF-a, IFNr, granulocyte colony stimulating factor (GCSF), granulocyte-macrophage colony-stimulating factor (GMCSF), IL-1a, IL-8, IP-10 and RANTES) using a commercial ELISA kit. The level of gene expression of CD-14, TLR-2, TLR-4, IL-10, IL-8, IL-2, INF-r, and TNF-a was higher in treated animals compared to control. The Sericea Lespedeza diet affected the secretion of pro-inflammatory cytokines by increasing the serum levels of TNF-a, IFNr, GCSF, GMCSF, IL-1a, IP-10 (P < 0.0002), and by decreasing (P < 0.0001) IL-8 and RANTES in blood from goats fed SL. This suggests that dietary tannins modulate gene expression and may affect the goat's innate immune response in blood. Further research is needed to understand and harness the effect of dietary condensed tannins to modulate innate immunity in goats.

Suggested Citation

  • Mulumebet Worku & Ahmed Abdalla & Sarah Adjei-Fremah & Hamid Ismail, 2016. "The Impact of Diet on Expression of Genes Involved in Innate Immunity in Goat Blood," Journal of Agricultural Science, Canadian Center of Science and Education, vol. 8(3), pages 1-1, February.
    • Handle: RePEc:ibn:jasjnl:v:8:y:2016:i:3:p:1
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    Cited by:

    1. Mulumebet Worku & Sarah Adjei-Fremah & Niki Whitley & Louis Jackai, 2017. "Effect of Cowpea (Vigna unguiculata) Pasture Grazing on Growth, Gastrointestinal Parasite Infection and Immune Response Biomarkers of Goat," Journal of Agricultural Science, Canadian Center of Science and Education, vol. 10(1), pages 1-27, December.

    More about this item

    JEL classification:

    • R00 - Urban, Rural, Regional, Real Estate, and Transportation Economics - - General - - - General
    • Z0 - Other Special Topics - - General

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