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Identification and Screening of Citrus Vein Phloem Degeneration (CVPD) on Brastagi Citrus Variety Brastepu (Citrus nobilis Brastepu) in North Sumatra Indonesia

Author

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  • Isnaini Nurwahyuni
  • Justin Napitupulu
  • Rosmayati Rosmayati
  • Fauziyah Harahap

Abstract

Identification and screening of Citrus Vein Phloem Degeneration (CVPD) on Brastagi citrus variety Brastepu (Citrus nobilis Brastepu) in North Sumatra Indonesia is explained. The aim of the study is to explore the technique to obtain healthy Brastepu citrus to be used as a source of explants for in vitro propagation as a step in the preservation of threatened local citrus. The studies were conducted through collection of survived Brastepu citrus followed by screening the CVPD in the plants. Various procedures in the field and in the laboratory have been conducted to screen infected CVPD in Brastepu citrus. Visual examination from leaf, fruit, and seed appearance becomes preliminary information for CVPD infection in the citrus plant. Iodine test was used to support results obtained by visual observation. Histochemical test and the PCR analysis have confirmed that 12 samples of Brastepu citrus are free from CVPD and eight citrus are infected by CVPD. Adequate informations have been obtained to confirm healthy citrus tree which are then to be used as sources of explants for in vitro propagation in the preservation of Citrus nobilis Brastepu.

Suggested Citation

  • Isnaini Nurwahyuni & Justin Napitupulu & Rosmayati Rosmayati & Fauziyah Harahap, 2015. "Identification and Screening of Citrus Vein Phloem Degeneration (CVPD) on Brastagi Citrus Variety Brastepu (Citrus nobilis Brastepu) in North Sumatra Indonesia," Journal of Agricultural Science, Canadian Center of Science and Education, vol. 7(4), pages 1-30, March.
    • Handle: RePEc:ibn:jasjnl:v:7:y:2015:i:4:p:30
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    References listed on IDEAS

    as
    1. khairulmazmi ahmad & Kamaruzaman Sijam & Habibuddin Hashim & Arifin Abdu & Zamri Rosli, 2011. "Assessment of Citrus Susceptibility towards Candidatus Liberibacter Asiaticus-Terengganu Isolate Based on Vector and Graft Transmission Tests," Journal of Agricultural Science, Canadian Center of Science and Education, vol. 3(3), pages 159-159, September.
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      JEL classification:

      • R00 - Urban, Rural, Regional, Real Estate, and Transportation Economics - - General - - - General
      • Z0 - Other Special Topics - - General

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