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Expression of the DRB1*1101 Allele in Meat Goats Pasture Exposed to Haemonchus contortus

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  • Michelle Corley
  • A. Savage

Abstract

The Major Histocompatibility class II complex plays an extremely important role in disease resistance and susceptibility. A mutation in the DRB1*1101 gene of the MHC II has been implicated in parasite resistance in sheep. Evaluation of the DRB1*1101 allele and the role it plays in Haemonchus contortus infected goats has not been addressed. This study evaluated expression of the DRB1*1101 allele in goats naturally infected with Haemonchus contortus infection. Total RNA was isolated from whole blood and RT-PCR (Reverse-Transcriptase Polymerase Chain Reaction) using primers designed to target the conserved regions of the human and sheep DRB1*1101 allele. The expected cDNA product was observed at the 201bp of the targeted DRB1*1101 region and verified by cloning and sequencing. Alignment of the Goat DRB1*1101 sequence showed 92% homology at the transcriptional level and 86% homology at the translational level with sheep. Goats pasture exposed to Haemonchus contortus had elevated (P < 0.05) DRB1*1101 allele expression. Expression was highest (P < 0.05) in Boer goats, lower in Myotonic goats and lowest (P < 0.05) in Spanish goats. Males had higher (P < 0.05) DRB1*1101 expression than females. DRB1*1101 gene expression was higher (P < 0.05) in younger vs. older goats. Goats showing resistance to Haemonchus contortus had lower (P < 0.05) DRB1*1101 expression than susceptible goats. Based on our findings, the DRB1*1101 gene in goats can potentially be used as a genetic marker for either resistance or susceptibility to Haemonchus contortus.

Suggested Citation

  • Michelle Corley & A. Savage, 2015. "Expression of the DRB1*1101 Allele in Meat Goats Pasture Exposed to Haemonchus contortus," Journal of Agricultural Science, Canadian Center of Science and Education, vol. 7(11), pages 1-19, October.
    • Handle: RePEc:ibn:jasjnl:v:7:y:2015:i:11:p:19
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    References listed on IDEAS

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    1. M. M. Corley & A. A. Jarmon, 2012. "A Common Beta Tubulin Isotype-1 Gene Single Nucleotide Polymorphism as a Tool for Detection and Quantitation of Anthelmintic Resistant Haemonchus contortus in Grazing Goats," Journal of Agricultural Science, Canadian Center of Science and Education, vol. 4(6), pages 1-1, April.
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      JEL classification:

      • R00 - Urban, Rural, Regional, Real Estate, and Transportation Economics - - General - - - General
      • Z0 - Other Special Topics - - General

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