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Kinetin Is the Most Effective Cytokinin on Shoot Multiplication from Cucumber

Author

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  • Saeid Abu-Romman
  • Khaldoun Al-Hadid
  • Abdullah Arabiyyat

Abstract

In vitro shoot multiplication of cucumber (Cucumis sativus) was examined from the nodal explants of 10-day-old aseptic plantlets using Murashige and Skoog (MS) media supplemented with different concentration (0, 0.5, 1, 2, 3 mg L-1) of cytokinins (6-Benzylaminopurine-BAP, Kinetin-Kn, Thidiazuron-TDZ, and Zeatin). Nodal explants of cucumber showed shoot induction and multiplication in response to all cytokinins tried. MS medium containing Kn was the most effective for inducing shoots from nodal explants of cucumber. The maximum rate of regeneration (83%), the highest number of obtained shoots (7.93 shoots/explant) and the longest shoots (3.61 cm) were obtained on MS medium fortified with 1 mg L-1 Kn. The lowest culture responses were recorded for media supplemented with either BAP or Zeatin. In conclusion, using Kn is strongly recommended than using Zeatin, TDZ and BAP to obtain the highest percentage of regeneration, the highest number of shoot/explant, and the highest shoot length for nodal explants of cucumber.

Suggested Citation

  • Saeid Abu-Romman & Khaldoun Al-Hadid & Abdullah Arabiyyat, 2015. "Kinetin Is the Most Effective Cytokinin on Shoot Multiplication from Cucumber," Journal of Agricultural Science, Canadian Center of Science and Education, vol. 7(10), pages 159-159, September.
    • Handle: RePEc:ibn:jasjnl:v:7:y:2015:i:10:p:159
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    JEL classification:

    • R00 - Urban, Rural, Regional, Real Estate, and Transportation Economics - - General - - - General
    • Z0 - Other Special Topics - - General

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