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Optimization of Saccharification Conditions of Acid-pretreated Sweet Sorghum Straw Using Response Surface Methodology

Author

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  • Sukanya Phuengjayaem
  • Aphisit Poonsrisawat
  • Amorn Petsom
  • Siriluk Teeradakorn

Abstract

This study focused on the cellulase production from C. versicolor TD17, white rot fungi. The maximum cellulase activity of 0.897 U/ml was obtained after 5 days of cultivation using 20 g/l cellobiose as a carbon source and 2 g/l ammonium sulfate supplemented with 0.3 g/l urea as nitrogen sources. Enzymatic saccharification of acid-pretreated sweet sorghum straw (SSS) using in house cellulase was optimized using Response Surface Methodology (RSM), variable five-code-level, four-factor; 1.0-7.0% w/v acid-pretreated SSS, 15-35 FPU/g dry substrate of cellulase enzyme, pH 3 to 7 and temperatures 30 to 70 °C. The optimal conditions were 1% w/v acid-pretreated SSS, 25 FPU/g dry substrate of cellulase, pH 5, 50 °C and 72 h cultivation. A maximal glucose yield of 0.440 g/g dry substrate was obtained.

Suggested Citation

  • Sukanya Phuengjayaem & Aphisit Poonsrisawat & Amorn Petsom & Siriluk Teeradakorn, 2014. "Optimization of Saccharification Conditions of Acid-pretreated Sweet Sorghum Straw Using Response Surface Methodology," Journal of Agricultural Science, Canadian Center of Science and Education, vol. 6(9), pages 120-120, August.
    • Handle: RePEc:ibn:jasjnl:v:6:y:2014:i:9:p:120
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    JEL classification:

    • R00 - Urban, Rural, Regional, Real Estate, and Transportation Economics - - General - - - General
    • Z0 - Other Special Topics - - General

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