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A Rapid and Efficient Method for the Isolation of Mitochondrial DNA From Wheat Crop

Author

Listed:
  • Muhammad Ejaz
  • Zhang Gaisheng
  • Zhu Qidi
  • Zhang Xinbo

Abstract

The mitochondrial DNA was isolated from wheat (Triticum aestivum) crop in combination of different centrifugation and density gradient ultracentrifugation, DNaseI enzyme treatment, sucrose sedimentation; lysis with SDS and potassium proteinase. Pure mitochondrial DNA was successfully obtained by phenol/chloroform/isoamyl alcohol extraction to remove protein, and digested with RNase phenol/chloroform extraction method. To detect mitochondrial DNA purity, specific primers were designed for nuclear (Beta-actin) and mitochondrial (COXII) gene. Isolated mitochondrial DNA is pure, suitable for PCR and genetic analysis.

Suggested Citation

  • Muhammad Ejaz & Zhang Gaisheng & Zhu Qidi & Zhang Xinbo, 2013. "A Rapid and Efficient Method for the Isolation of Mitochondrial DNA From Wheat Crop," Journal of Agricultural Science, Canadian Center of Science and Education, vol. 5(6), pages 1-32, May.
    • Handle: RePEc:ibn:jasjnl:v:5:y:2013:i:6:p:32
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    More about this item

    JEL classification:

    • R00 - Urban, Rural, Regional, Real Estate, and Transportation Economics - - General - - - General
    • Z0 - Other Special Topics - - General

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