Measurement of Lipid Peroxidation and Phenol Contents Under Heat Stress Condition in Sugarcane Genotypes

The experiment conducted in the laboratory of Plant Breeding and Molecular Genetics, Faculty of Agriculture, Rawalakot, Pakistan. Leaves of thirteen genotypes of sugarcane used for this experiment and were treated in an oven for heat stress. The treated leaves were then used for the estimation of phenol contents and the measurement of lipid peroxidation against heat stress. Minimum moisture loosed by CPF-237 and maximum moisture loss showed by CO-1148. Maximum phenol contents were observed in S-2003-US-778 showed high results (58.78 mg GAE/100ml) and minimum were Rb-72 and CO-1148 showed the lowest values (36.17 and 37.5 mg GAE/100ml). The higher heat tolerance of genotype, CPF-237 related to higher membrane stability, and maintenance of high fv/fm ratio under heat stress and lower lipid peroxidation of membranes. Hence, the relative tolerance of a genotype to heat stress as reflected by its lower lipid peroxidation, and higher membrane stability and pigment concentration, related to the levels of activity of its antioxidant enzymes. By using morphological parameters, S-2003-US-778 was significantly different from Rb-72. Based on biochemical data, the genotypes S-2003-US-778, S-2003-US-694, CPF-237 and Co-1148 are heat tolerant genotypes and thus can be successfully planted in warmer areas to alleviate the socioeconomic status of farmers.