Author
Listed:
- Tarek Shalaby
- Antar El-Banna
Abstract
In addition to the traditional breeding approaches, genetic variability in tomato can be induced also by means of mutagenesis. The objective of this study was to develop an In vitro technique suitable for mutation induction on tomato and characterize them by RAPD and SSR markers as well as horticultural characteristics. The influence of various concentrations (0, 0.07, 0.14 and 0.25%) of the chemical mutagen, Ethyl Methane Sulfonate (EMS), on the in vitro shoot formation from cotyledon explants of two tomato cultivars was studied. The percentage of responding explants ranged from 45.2 to 95% in dependence on genotype and EMS concentrations. Two PCR-based techniques, RAPD and SSR, were used for analysis of genetic variations in regenerated plants from in vitro cultures combined with EMS treatment (0.25%). The percentage of polymorphism detected by RAPD and SSR primers reached 25.64%. Grouping of the original cultivar and their mutants indicated the genetic distinctness as they are placed in different clusters/groups far from each other. Mutants regenerated from the wide cultivated cultivar in Egypt (Super strain B) were evaluated with their origin cultivar in a field experiment for yield potential and fruit quality. The results revealed that the mutants were differed in number of branches, early and total yield, average fruit weight, fruit firmness and TSS content. Moreover, mutant lines S1, S3, S6 and S13 had some desirable horticultural traits and could be used in improving tomato crop by breeding programs or they could be considered as new breeding lines.
Suggested Citation
Tarek Shalaby & Antar El-Banna, 2013.
"Molecular and Horticultural Characteristics of In vitro Induced Tomato Mutants,"
Journal of Agricultural Science, Canadian Center of Science and Education, vol. 5(10), pages 155-155, September.
Handle:
RePEc:ibn:jasjnl:v:5:y:2013:i:10:p:155
Download full text from publisher
More about this item
JEL classification:
- R00 - Urban, Rural, Regional, Real Estate, and Transportation Economics - - General - - - General
- Z0 - Other Special Topics - - General
Statistics
Access and download statistics
Corrections
All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:ibn:jasjnl:v:5:y:2013:i:10:p:155. See general information about how to correct material in RePEc.
If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.
We have no bibliographic references for this item. You can help adding them by using this form .
If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.
For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Canadian Center of Science and Education (email available below). General contact details of provider: https://edirc.repec.org/data/cepflch.html .
Please note that corrections may take a couple of weeks to filter through
the various RePEc services.