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PCR-RFLP Analysis of cpDNA in Tea Cultivars (Camellia sinensis L.) in Sichuan of China

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Listed:
  • Shengxiang Chen
  • Guinian Qi
  • Huan Li
  • Yao Zou
  • Hongli Shan

Abstract

The genetic diversity among 30 tea cultivars in Sichuan of China was investigated by PCR-RFLP analysis of cp DNA. 7 sets of chloroplast primers could produce one or more than one distinct bands by direct electrophoresis in 2% agrose gels. After the amplified products were digested by 10 restriction enzymes, a total of 135 bands were detected, among which 98 bands (72.59%) were polymorphic. The cpDNA PCR-RFLP based genetic distance (GD) among 30 tea accessions ranged from 0 to 0.071, with the mean of 0.049.

Suggested Citation

  • Shengxiang Chen & Guinian Qi & Huan Li & Yao Zou & Hongli Shan, 2012. "PCR-RFLP Analysis of cpDNA in Tea Cultivars (Camellia sinensis L.) in Sichuan of China," Journal of Agricultural Science, Canadian Center of Science and Education, vol. 4(5), pages 1-25, March.
  • Handle: RePEc:ibn:jasjnl:v:4:y:2012:i:5:p:25
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    JEL classification:

    • R00 - Urban, Rural, Regional, Real Estate, and Transportation Economics - - General - - - General
    • Z0 - Other Special Topics - - General

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