IDEAS home Printed from https://ideas.repec.org/a/ibn/jasjnl/v4y2012i12p243.html
   My bibliography  Save this article

Cloning and Characterization of a NBS-LRR Resistance Gene from Peanut (Arachis hypogaea L.?

Author

Listed:
  • Yi-xiong Zheng
  • Chun-juan Li
  • Yu Liu
  • Cai-xia Yan
  • Ting-ting Zhang
  • Wei-jian Zhuang
  • Shi-hua Shan

Abstract

The nucleotide-binding site (NBS)-Leucine-rich repeat (LRR) gene family accounts for the largest number of known disease resistance genes, and is one of the largest gene families in plant genomes. In the present study, based on the NBS domain, resistance gene analogues (RGAs) have been isolated from peanut, which named PnAG3. A full-length cDNA, PnAG3 was obtained by rapid amplification of cDNA ends (RACE) method. Sequence analysis indicated that the length of PnAG3 was 1 882 bp, including a complete open reading frame of 1 335 bp encoding PnAG3 protein of 444 amino acids. Multiple analysis showed that it had a certain homology with known resistance proteins, among which Arachis cardenasii resistance protein had the highest homology (48.01%). The polypeptide has a typical structure of nonTIR-NBS-LRR genes. Real-time fluorescence quantitative PCR analysis showed that after A. flavus infection, the expression of PnAG3 gene in J11 (A. flavus resistance species) has increased 16.68, 11.16 and 25.96 times in seed coat, kernel and pericarp, respectively. But it only increased 2-3 times in JH1012 (A. flavus sensitive species). The cloning of putative resistance gene from peanut provides a basis for studying the structure and function of peanut disease-resistance relating genes and disease resistant genetic breeding in peanut.

Suggested Citation

  • Yi-xiong Zheng & Chun-juan Li & Yu Liu & Cai-xia Yan & Ting-ting Zhang & Wei-jian Zhuang & Shi-hua Shan, 2012. "Cloning and Characterization of a NBS-LRR Resistance Gene from Peanut (Arachis hypogaea L.?," Journal of Agricultural Science, Canadian Center of Science and Education, vol. 4(12), pages 243-243, November.
    • Handle: RePEc:ibn:jasjnl:v:4:y:2012:i:12:p:243
    as

    Download full text from publisher

    File URL: https://ccsenet.org/journal/index.php/jas/article/download/19158/14316
    Download Restriction: no
    File URL: https://ccsenet.org/journal/index.php/jas/article/view/19158
    Download Restriction: no
    ---><---

    More about this item

    JEL classification:

    • R00 - Urban, Rural, Regional, Real Estate, and Transportation Economics - - General - - - General
    • Z0 - Other Special Topics - - General

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:ibn:jasjnl:v:4:y:2012:i:12:p:243. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Canadian Center of Science and Education (email available below). General contact details of provider: https://edirc.repec.org/data/cepflch.html .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.