Evaluation of the Immunomodulatory Activities for Ethyl Acetate Fraction of Cynodon dactylon in Balb/c mice

The immunomodulatory properties of ethyl acetate fraction of aqueous extract of Cynodon dactylon have been investigated in experimental animal as Balb/c mice. In this present study, Balb/c male mice of 5-7 weeks old (20-25g) were maintained under standard laboratory conditions (temperature 25 ± 2°C) with dark/light cycle (14/10h). The mice were acclimatized to laboratory conditions for 15 days before the commencement of the experiments. The mice were divided into two groups and each group containing 6 mice. The present investigation has revealed that pyrogallol at 50 mg/kg body weight, produced significant impairment of humoral as well as cell-mediated immune responses. It was observed that daily treatment of 70ul of ethyl acetate fraction of Cynodon dactylon 34.3ug polyphenols significantly prevented the immunosuppression caused by pyrogallol. This result suggested that the immunomodulatory effect of ethyl acetate fraction of Cynodon dactylon can be screened by the method in which the immunosuppression was induced by pyrogallol. Hence, we expect that ethyl acetate fraction of aqueous extracts of Cynodon dactylon has strong utility in clinical practice as an effective immunostimulant.