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Molecular Characterization and Evolutionary Analysis of Alpha-gliadin Genes from Eremopyrum bonaepartis (Triticeae)

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  • Guangrong Li
  • Tao Zhang
  • Yirong Ban
  • Zujun Yang

Abstract

Total 16 ?-gliadin gene sequences ranged from 1186 to 1316bp were isolated from Eremopyrum bonaepartis byPCR based strategy. Analysis of deduced amino acid sequences indicated that 8 of 16 sequences displayed thetypical structure of ?-gliadin genes with six cysteine residues, while the other 8 sequences contained in-framestop codon, and were therefore pseudogenes. Phylogenetic analysis based on the variation of ?-gliadin genesequences indicated that the F genome of E. bonaepartis was apparently differed from the A, B and D genomesof wheat. Four peptides, Glia-?, Glia-?2, Glia-?9 and Glia-?20, which have been identified as T cell stimulatoryepitopes in celiac disease (CD) patients through binding to HLA-DQ2/8, were searched to the E. bonaepartis?-gliadin gene sequences. We firstly found that only Glia-? existed in 7 of 8 full sequences, the fact suggestingthat the occurrence of the stimulatory epitopes in ?-gliadin genes was newly evolved in wheat.

Suggested Citation

  • Guangrong Li & Tao Zhang & Yirong Ban & Zujun Yang, 2010. "Molecular Characterization and Evolutionary Analysis of Alpha-gliadin Genes from Eremopyrum bonaepartis (Triticeae)," Journal of Agricultural Science, Canadian Center of Science and Education, vol. 2(4), pages 1-30, November.
    • Handle: RePEc:ibn:jasjnl:v:2:y:2010:i:4:p:30
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    JEL classification:

    • R00 - Urban, Rural, Regional, Real Estate, and Transportation Economics - - General - - - General
    • Z0 - Other Special Topics - - General

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