IDEAS home Printed from https://ideas.repec.org/a/ibn/jasjnl/v12y2024i12p192.html
   My bibliography  Save this article

Seed Viability and RNA Extraction Methods of Erythrina velutina Under Drought Stress

Author

Listed:
  • Juliana L. Souza
  • Bruno A. L. de Freitas
  • Laura C. D. P. Lima
  • Valdinete V. Nunes
  • Maria F. O. Torres
  • Renata Silva-Mann

Abstract

Abiotic stress caused by rigorous environmental conditions, such as drought, has negative effects on seeds. The species from arid and semiarid areas have mechanisms to maintain seed viability. In this study, the analyses of physical and physiological quality of the seeds of Erythrina velutina and their response to drought stress and RNA extraction methods were carried out. The seeds were collected from mother trees in two different provenances of natural occurrences of the species. The physical quality, viability, and vigor of the seeds were evaluated by morphometric parameters, germination, and electrical conductivity tests. The seeds were submitted to drought stress conditions for 0, 24, 48, and 72 hours on -0.8 MPa polyethylene glycol solution. To evaluate the viable RNA extraction method, the seed embryos were used for the extraction of this molecule and with an assessment of quantity and quality. The seeds from Pernambuco that were harvested in 2008 were bigger and presented lower vigor when in comparison to seeds that were harvested from Sergipe in 2012. The seeds under drought stress conditions presented null germination for all the treatments. After the drought stress, they were germinated on a paper substrate moistened with water, with better responses from the Lot Sergipe, 2012. The imbibition of seeds in the solution for 24 hours provides an increase in germination for less vigorous seeds, being indicated as a pre-germinative treatment. The RNA quality obtained by using a commercial extraction kit has better results, providing materials without contaminants when compared to the TRIzol method.

Suggested Citation

  • Juliana L. Souza & Bruno A. L. de Freitas & Laura C. D. P. Lima & Valdinete V. Nunes & Maria F. O. Torres & Renata Silva-Mann, 2024. "Seed Viability and RNA Extraction Methods of Erythrina velutina Under Drought Stress," Journal of Agricultural Science, Canadian Center of Science and Education, vol. 12(12), pages 192-192, April.
  • Handle: RePEc:ibn:jasjnl:v:12:y:2024:i:12:p:192
    as

    Download full text from publisher

    File URL: https://ccsenet.org/journal/index.php/jas/article/download/0/0/44161/46498
    Download Restriction: no

    File URL: https://ccsenet.org/journal/index.php/jas/article/view/0/44161
    Download Restriction: no
    ---><---

    More about this item

    JEL classification:

    • R00 - Urban, Rural, Regional, Real Estate, and Transportation Economics - - General - - - General
    • Z0 - Other Special Topics - - General

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:ibn:jasjnl:v:12:y:2024:i:12:p:192. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Canadian Center of Science and Education (email available below). General contact details of provider: https://edirc.repec.org/data/cepflch.html .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.