IDEAS home Printed from https://ideas.repec.org/a/ibn/jasjnl/v11y2024i6p519.html
   My bibliography  Save this article

Molecular Characterization of Isolates of Fusarium spp. Associated With Wilt in Capsicum spp

Author

Listed:
  • Isabela V. dos Anjos
  • Suelene S. de Melo
  • Thiago A. S. Gilio
  • Jessé P. Kreitlow
  • Sandra M. A. da S. Neves
  • Kelly L. Araújo
  • Milson E. Serafim
  • Leonarda G. Neves

Abstract

Fusarium is a diverse and heterogeneous fungi genus. Its wide genetic variability and similarity in morphological characteristics hinder the identification of species of this genus. Identifying Fusarium species is difficult due to the genus. Several molecular methods have been useful for differentiating these species, and the amplification of internal transcribed spacer (ITS) regions of the fungus ribosomal DNA has been successfully used, since ITS are preserved regions of the DNA that assists in distinguishing species. The objective of this work was to collect and characterize isolates of Fusarium spp. associated with wilt symptoms in Capsicum spp. in the biomes of the state of Mato Grosso, Brazil. Were collected tissue samples of plants with wilt symptoms. The DNAs of the Fusarium spp. found were extracted, and subjected to polymerase chain reaction, using the primers ITS1 (5’TCCGTAGGTGAACCTGCGG-3’) and ITS4 (5’-TCCTCCGCTTATTGATATGC-3’). Subsequently, the sequencing was performed. The resulting sequences were, five Fusarium species were found F. solani, F. oxysporum, F. equiseti, F. incarnatum, F. chlamydosporum, predominating F. solani and F. equiseti.

Suggested Citation

  • Isabela V. dos Anjos & Suelene S. de Melo & Thiago A. S. Gilio & Jessé P. Kreitlow & Sandra M. A. da S. Neves & Kelly L. Araújo & Milson E. Serafim & Leonarda G. Neves, 2024. "Molecular Characterization of Isolates of Fusarium spp. Associated With Wilt in Capsicum spp," Journal of Agricultural Science, Canadian Center of Science and Education, vol. 11(6), pages 519-519, April.
  • Handle: RePEc:ibn:jasjnl:v:11:y:2024:i:6:p:519
    as

    Download full text from publisher

    File URL: https://ccsenet.org/journal/index.php/jas/article/download/0/0/39292/40123
    Download Restriction: no

    File URL: https://ccsenet.org/journal/index.php/jas/article/view/0/39292
    Download Restriction: no
    ---><---

    More about this item

    JEL classification:

    • R00 - Urban, Rural, Regional, Real Estate, and Transportation Economics - - General - - - General
    • Z0 - Other Special Topics - - General

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:ibn:jasjnl:v:11:y:2024:i:6:p:519. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Canadian Center of Science and Education (email available below). General contact details of provider: https://edirc.repec.org/data/cepflch.html .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.