Author
Listed:
- Leyza Paloschi de Oliveira
- Cassio Geremia Freire
- Simone Silmara Werner
- Mari Inês Carissimi Boff
- Pedro Boff
Abstract
In vitro propagation of plants makes it possible to accelerate the process of plant multiplication, the study of secondary metabolite production and the cultivation of biotrophic fungi. The objective of this work was to study the combination of indoleacetic acid (IAA) and benzylaminopurine (BAP) and explant sizes in in vitro multiplication of M. sylvestris. Five concentrations of BAP (0, 0.5, 1.0, 1.5 and 2.0 mg L-1) and two of IAA (0 and 0.5 mg L-1) were used in explants of 4 to 9 mm and of 14 to 23 mm. Contaminated explants, oxidation, establishment, relative growth rate (RGR), sprouting, rooting and callus formation were evaluated. There was no interaction effect between BAP and IAA concentrations. At 28 days, explants were established at 32.76% and callus formation was 62.5% for explants associated with 0.0 mg L-1 of IAA. There was 32.14% establishment and 63.79% callus formation at 0.5 mg L-1 of IAA. Bacterial contamination at 28 days was 60.53%, twice as much the amount found at 14 days, suggesting that the explants presented endogenous contamination. It was found that explant size influences the subsequent meristematic development. The use of smaller explants (4 to 9 mm) allowed greater formation of calli and larger explants (14 to 23 mm) allowed greater formation of shoots. In conclusion, larger explants are preferable for production of M. sylvestris in vitro by organogenesis while smaller ones are preferable for embryogenesis.
Suggested Citation
Leyza Paloschi de Oliveira & Cassio Geremia Freire & Simone Silmara Werner & Mari Inês Carissimi Boff & Pedro Boff, 2024.
"Phytoregulators and Explant Size in the in vitro Culture of Malva sylvestris,"
Journal of Agricultural Science, Canadian Center of Science and Education, vol. 11(1), pages 388-388, April.
Handle:
RePEc:ibn:jasjnl:v:11:y:2024:i:1:p:388
Download full text from publisher
More about this item
JEL classification:
- R00 - Urban, Rural, Regional, Real Estate, and Transportation Economics - - General - - - General
- Z0 - Other Special Topics - - General
Statistics
Access and download statistics
Corrections
All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:ibn:jasjnl:v:11:y:2024:i:1:p:388. See general information about how to correct material in RePEc.
If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.
We have no bibliographic references for this item. You can help adding them by using this form .
If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.
For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Canadian Center of Science and Education (email available below). General contact details of provider: https://edirc.repec.org/data/cepflch.html .
Please note that corrections may take a couple of weeks to filter through
the various RePEc services.