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Viability of Carica papaya L. Seeds by the Tetrazolium Test

Author

Listed:
  • Sara Carvalho
  • Salvador Torres
  • Erivanessa Sousa
  • Danielle Sousa
  • Kleane Pereira
  • Emanoela Paiva
  • Janete Matias
  • Brenna Santos

Abstract

Germination test for papaya seeds demands thirty days to obtain the results, which is a long period for decision-making. Thus, rapid tests become essential tools to evaluate seed viability, and the tetrazolium test is an alternative for this purpose. Hence, this study aimed to establish the adequate procedure to apply this test, in order to reduce the time of evaluation of papaya seeds. For that, four tetrazolium solution concentrations (0.05, 0.1, 0.5 and 1%) and three staining times (3, 6 and 9 hours) were tested at temperatures of 35 and 40 ºC. The experimental design was completely randomized in 4 × 3 + 1 factorial scheme (concentrations × staining times + one control = germination test). It was found that the tetrazolium test allows to evaluate the physiological quality of papaya seeds, thus reducing the time for decision-making. For the test, we recommend using the concentrations of 0.1% for 9 hours or 1% for 6 hours of staining, at temperature of 40 ºC.

Suggested Citation

  • Sara Carvalho & Salvador Torres & Erivanessa Sousa & Danielle Sousa & Kleane Pereira & Emanoela Paiva & Janete Matias & Brenna Santos, 2018. "Viability of Carica papaya L. Seeds by the Tetrazolium Test," Journal of Agricultural Science, Canadian Center of Science and Education, vol. 10(2), pages 335-335, January.
    • Handle: RePEc:ibn:jasjnl:v:10:y:2018:i:2:p:335
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    JEL classification:

    • R00 - Urban, Rural, Regional, Real Estate, and Transportation Economics - - General - - - General
    • Z0 - Other Special Topics - - General

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