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Carbazole Degradation and Genetic Analyses of Sphingobium sp. Strain BS19 Isolated from Antarctic Soil

Author

Listed:
  • Kenta Sato

    (Department of Bioscience and Engineering, College of Systems Engineering and Science, Shibaura Institute of Technology, 307 Fukasaku, Minuma-ku, Saitama 337-8570, Japan)

  • Seiryu Take

    (Department of Bioscience and Engineering, College of Systems Engineering and Science, Shibaura Institute of Technology, 307 Fukasaku, Minuma-ku, Saitama 337-8570, Japan)

  • Siti Aqlima Ahmad

    (Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia)

  • Claudio Gomez-Fuentes

    (Center for Research and Antarctic Environmental Monitoring (CIMAA), Universidad de Magallanes, Avda, Bulnes 01855, Punta Arenas 6210427, Chile)

  • Azham Zulkharnain

    (Department of Bioscience and Engineering, College of Systems Engineering and Science, Shibaura Institute of Technology, 307 Fukasaku, Minuma-ku, Saitama 337-8570, Japan)

Abstract

The Antarctic region is facing a higher risk of hydrocarbon pollution due to increased human activities. Compounds such as polycyclic aromatic hydrocarbons (PAHs) and heterocyclic compounds available in fuel are highly stable and can reside in the environment for prolonged periods if left untreated. The isolation of native strains is needed to develop bioremediation applications suitable for Antarctica. Strain BS19 was isolated as heterocyclic compound carbazole-degrading bacterium from Antarctic soil through culture enrichment. The 16S rRNA gene sequences identified strain BS19 as a member of the Sphingonium genus. Strain BS19 could remove 75% of carbazole after 15 days of culture at 15 °C. Whole genome sequencing resulted in incomplete genomes of 4.77 Mb in 96 contigs with the lowest GC content among Sphingobium sp. strains. The analyses revealed car gene cluster and ant genes and cat gene cluster required for the complete metabolism of carbazole as a source of carbon and energy. The comparison of the car gene cluster showed a similarity to the car gene cluster of Novosphingobium KA1. The expression of the car gene cluster was confirmed with an RT-PCR analysis indicating the involvement of the predicted genes in carbazole degradation. The findings from this study could provide more insight into developing bioremediation applications and approaches for Antarctica and other cold environments.

Suggested Citation

  • Kenta Sato & Seiryu Take & Siti Aqlima Ahmad & Claudio Gomez-Fuentes & Azham Zulkharnain, 2023. "Carbazole Degradation and Genetic Analyses of Sphingobium sp. Strain BS19 Isolated from Antarctic Soil," Sustainability, MDPI, vol. 15(9), pages 1-13, April.
    • Handle: RePEc:gam:jsusta:v:15:y:2023:i:9:p:7197-:d:1133269
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    References listed on IDEAS

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    1. Gayathiri Verasoundarapandian & Chiew-Yen Wong & Noor Azmi Shaharuddin & Claudio Gomez-Fuentes & Azham Zulkharnain & Siti Aqlima Ahmad, 2021. "A Review and Bibliometric Analysis on Applications of Microbial Degradation of Hydrocarbon Contaminants in Arctic Marine Environment at Metagenomic and Enzymatic Levels," IJERPH, MDPI, vol. 18(4), pages 1-30, February.
    2. Ahmad Fareez Ahmad Roslee & Siti Aqlima Ahmad & Claudio Gomez-Fuentes & Noor Azmi Shaharuddin & Khalilah Abdul Khalil & Azham Zulkharnain, 2021. "Scientometric Analysis of Diesel Pollutions in Antarctic Territories: A Review of Causes and Potential Bioremediation Approaches," Sustainability, MDPI, vol. 13(13), pages 1-24, June.
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