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COS Attenuates AFB 1 -Induced Liver Injury in Medaka through Inhibition of Histopathological Damage and Oxidative Stress

Author

Listed:
  • Huijun Shi

    (College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306, China)

  • Lin Chen

    (College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306, China)

  • Zhaohuan Zhang

    (College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306, China)

  • Yong Zhao

    (College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306, China
    Shanghai Engineering Research Center of Aquatic Product Processing & Preservation, Shanghai 201306, China
    Laboratory of Quality and Safety Risk Assessment for Aquatic Product on Storage and Preservation, Ministry of Agriculture and Rural Affairs, Shanghai 201306, China)

  • Jie Ou

    (College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306, China
    Shanghai Engineering Research Center of Aquatic Product Processing & Preservation, Shanghai 201306, China
    Laboratory of Quality and Safety Risk Assessment for Aquatic Product on Storage and Preservation, Ministry of Agriculture and Rural Affairs, Shanghai 201306, China)

Abstract

Aflatoxin B1 (AFB 1 ) –induced liver damage may be treated with chitosan oligosaccharide (COS), a small-molecular–weight oligosaccharide with excellent bioactivity and antioxidant potential. Hepatotoxicity induced by AFB 1 single acute exposure (ASAE) has been theoretically established but the mechanism of toxicity in aquatic models has been less studied. In this paper, a model of liver injury in Japanese medaka ( Oryzias latipes ) after ASAE for 72 h and a model of liver injury healing after ASAE following a COS intervention for 72 h were developed. The different effects of ASAE and COS interventions for ASAE were analyzed at the phenotypic and genetic levels. The results showed that AFB 1 reduced body weight and hepatopancreatic somatic indices (HSI) in medaka. Moreover, AFB 1 –induced histopathological damage and oxidative stress injury were concentration–dependent but the symptoms of damage were attenuated to some extent by the addition of the intervention drug COS, and the intervention effect of high concentrations of COS was almost identical to silymarin (SIL). Using the RNA–Seq technique, COS reduces the number of differentially expressed genes (DEGs) brought about by AFB 1 . Among the genes associated with tumors, hepatocellular carcinoma and hepatitis aurka , thbs1 , serpine1 , fabp7 , and dusp5 were also validated by Q-PCR with corresponding trends. In conclusion, AFB 1 can cause liver injury in medaka and COS has a therapeutic effect, and these impacted genes have the potential to become therapeutic targets for COS intervention in AFB 1 –induced liver disease.

Suggested Citation

  • Huijun Shi & Lin Chen & Zhaohuan Zhang & Yong Zhao & Jie Ou, 2023. "COS Attenuates AFB 1 -Induced Liver Injury in Medaka through Inhibition of Histopathological Damage and Oxidative Stress," Sustainability, MDPI, vol. 15(6), pages 1-12, March.
    • Handle: RePEc:gam:jsusta:v:15:y:2023:i:6:p:5418-:d:1101215
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    More about this item

    Keywords

    aflatoxin B1; chitosan oligosaccharide; oxidative stress; histopathological; differentially expressed genes;
    All these keywords.

    JEL classification:

    • B1 - Schools of Economic Thought and Methodology - - History of Economic Thought through 1925

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