IDEAS home Printed from https://ideas.repec.org/a/gam/jsusta/v15y2023i5p4025-d1077078.html
   My bibliography  Save this article

In Vitro Seed and Clonal Propagation of the Mediterranean Bee Friendly Plant Anthyllis hermanniae L

Author

Listed:
  • Aikaterini N. Martini

    (Laboratory of Floriculture and Landscape Architecture, Department of Crop Science, School of Plants Sciences, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece)

  • Maria Papafotiou

    (Laboratory of Floriculture and Landscape Architecture, Department of Crop Science, School of Plants Sciences, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece)

Abstract

Anthyllis hermanniae L. (Fabaceae) is a perennial Mediterranean shrub with the potential to be used as a bee-friendly ornamental plant in arid and semi-arid regions, valued for its tolerance of barren soils, winds, and strong temperature changes. With the aim of facilitating the introduction of the species into the horticulture industry, its in vitro seed and clonal propagation was investigated for the first time, to our knowledge. Seeds stored in the dark at room temperature for 4, 7, 9, 12, and 18 months germinated at percentages higher than 80% after scarification, when incubated in vitro in solid half-strength Murashige and Skoog (MS) medium at temperatures from 10 to 25 °C, while photoperiod (continuous darkness or 16 h light period/8 h dark) during incubation did not affect germination. Explants excised from in vitro grown seedlings established at higher percentages compared to explants from adult native plants, more efficiently in MS medium with 1.0 mg L −1 6 N benzyladenine (BA). During subcultures in the same medium, juvenile explants formed more and longer shoots than adult ones. Almost all adult explants formed shoots when subcultured in MS medium with 0.0 to 4.0 mg L −1 BA, zeatin, kinetin or 6-(γ,γ-dimethylallylamino)purine (2iP). ΒA at 0.5 to 2.0 mg L −1 induced many more shoots (17–21) per explant and much higher multiplication indices compared to all other cytokinins, while longer shoots were produced in a medium without hormones or with 0.5–1.0 mg L −1 2iP. Microshoots cultured in half-strength MS medium with 0.0–4.0 mg L −1 indole-3-butyric acid rooted at highest percentage (around 70%) in the medium containing 4.0 mg L −1 IBA, while microshoots of juvenile origin developed more and longer roots compared to adult ones. Micropropagated plantlets were successfully acclimatized ex vitro (>97%), regardless of their origin. The efficient micropropagation of A. hermaniae will facilitate its sustainable exploitation as a bee-friendly landscape plant, a forage plant for honeybees in Mediterranean areas, and a medicinal plant.

Suggested Citation

  • Aikaterini N. Martini & Maria Papafotiou, 2023. "In Vitro Seed and Clonal Propagation of the Mediterranean Bee Friendly Plant Anthyllis hermanniae L," Sustainability, MDPI, vol. 15(5), pages 1-20, February.
    • Handle: RePEc:gam:jsusta:v:15:y:2023:i:5:p:4025-:d:1077078
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2071-1050/15/5/4025/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/2071-1050/15/5/4025/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Monika Egerer & Jacob M. Cecala & Hamutahl Cohen, 2019. "Wild Bee Conservation within Urban Gardens and Nurseries: Effects of Local and Landscape Management," Sustainability, MDPI, vol. 12(1), pages 1-19, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Arianna Latini & Ilaria Papagni & Lorenzo Gatti & Patrizia De Rossi & Alessandro Campiotti & Germina Giagnacovo & Daniele Mirabile Gattia & Susanna Mariani, 2022. "Echium vulgare and Echium plantagineum : A Comparative Study to Evaluate Their Inclusion in Mediterranean Urban Green Roofs," Sustainability, MDPI, vol. 14(15), pages 1-19, August.
    2. Martin Šlachta & Tomáš Erban & Alena Votavová & Tomáš Bešta & Michal Skalský & Marta Václavíková & Taťána Halešová & Magda Edwards-Jonášová & Renata Včeláková & Pavel Cudlín, 2020. "Domestic Gardens Mitigate Risk of Exposure of Pollinators to Pesticides—An Urban-Rural Case Study Using a Red Mason Bee Species for Biomonitoring," Sustainability, MDPI, vol. 12(22), pages 1-17, November.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jsusta:v:15:y:2023:i:5:p:4025-:d:1077078. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.