IDEAS home Printed from https://ideas.repec.org/a/gam/jsusta/v14y2022i22p15341-d977033.html
   My bibliography  Save this article

Enhancement of Wheat Growth by UV-Mutagenesis of Potential Chromium Tolerant Bacillus sp. Strain SR-2-1/1

Author

Listed:
  • Muhammad Shahid

    (Department of Bioinformatics & Biotechnology, Government College University Faisalabad, Faisalabad 38040, Pakistan)

  • Muhammad Usman

    (Department of Bioinformatics & Biotechnology, Government College University Faisalabad, Faisalabad 38040, Pakistan)

  • Tanvir Shahzad

    (Department of Environmental Sciences, Government College University Faisalabad, Faisalabad 38040, Pakistan)

  • Iftikhar Ali

    (State Key Laboratory of Molecular Development Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences (CAS), Beijing 100101, China
    Center for Plant Sciences and Biodiversity, University of Swat, Charbagh, Swat 19120, Pakistan)

  • Muhammad Umair Hassan

    (Research Center on Ecological Sciences, Jiangxi Agricultural University, Nanchang 330045, China)

  • Faisal Mahmood

    (Department of Environmental Sciences, Government College University Faisalabad, Faisalabad 38040, Pakistan)

  • Sameer H. Qari

    (Department of Biology, Al-Jumum University College, Umm Al-Qura University, Makkah 21955, Saudi Arabia)

Abstract

UV mutagenesis has long been known to improve bacterial strains in their physiological capacity. In the current study, we used the UV mutagenesis approach to increase the PGPR characteristics of a pre-characterized metal-tolerant PGPR strain Bacillus sp. strain SR-2-1/1 (KY315919), with the objective of increasing the physiological outcome of its PGPR traits in vitro and post inoculation on wheat plants. After UV irradiation, the results implied a substantial in vitro increase in the phosphate solubilization and ammonia production of two selected mutant strains (M 1 and M 2 ) as compared to the wild-type strain SR-2-1/1; however, the ACC deaminase enzyme activity was completely lost in the mutant strains, which were originally present in the wild-type strains. However, the UV mutagenesis did not affect the taxonomy of these mutant strains. Moreover, the mutant strains M 1 and M 2 survived in the rhizosphere of wheat plants up to 30 days at an optimum (approximately 7–7.5 Log CFU/mL of rhizosphere soil) population density. The fresh and dry biomass, as well as root and shoot length, of wheat plants inoculated with one of the mutant strains M 2 were significantly higher than in the wheat plants inoculated with wild-type strain SR-2-1/1. The overall results imply that the resulted mutant M 2 was a physiologically competent PGPR strain, which could be tested in field experiments as an inoculum.

Suggested Citation

  • Muhammad Shahid & Muhammad Usman & Tanvir Shahzad & Iftikhar Ali & Muhammad Umair Hassan & Faisal Mahmood & Sameer H. Qari, 2022. "Enhancement of Wheat Growth by UV-Mutagenesis of Potential Chromium Tolerant Bacillus sp. Strain SR-2-1/1," Sustainability, MDPI, vol. 14(22), pages 1-13, November.
    • Handle: RePEc:gam:jsusta:v:14:y:2022:i:22:p:15341-:d:977033
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2071-1050/14/22/15341/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/2071-1050/14/22/15341/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Muhammad Irfan Ahamad & Jinxi Song & Haotian Sun & Xinxin Wang & Muhammad Sajid Mehmood & Muhammad Sajid & Ping Su & Asif Jamal Khan, 2020. "Contamination Level, Ecological Risk, and Source Identification of Heavy Metals in the Hyporheic Zone of the Weihe River, China," IJERPH, MDPI, vol. 17(3), pages 1-17, February.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Marcin Sidoruk, 2023. "Pollution and Potential Ecological Risk Evaluation of Heavy Metals in the Bottom Sediments: A Case Study of Eutrophic Bukwałd Lake Located in an Agricultural Catchment," IJERPH, MDPI, vol. 20(3), pages 1-15, January.
    2. Kun Zhang & Bo Peng & Xia Yang, 2023. "Contamination and Risk of Heavy Metals in Sediments from Zhuzhou, Xiangtan and Changsha Sections of the Xiangjiang River, Hunan Province of China," Sustainability, MDPI, vol. 15(19), pages 1-20, September.
    3. Marioara Nicoleta Filimon & Ion Valeriu Caraba & Roxana Popescu & Gabi Dumitrescu & Doina Verdes & Liliana Petculescu Ciochina & Adrian Sinitean, 2021. "Potential Ecological and Human Health Risks of Heavy Metals in Soils in Selected Copper Mining Areas—A Case Study: The Bor Area," IJERPH, MDPI, vol. 18(4), pages 1-18, February.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jsusta:v:14:y:2022:i:22:p:15341-:d:977033. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.