IDEAS home Printed from https://ideas.repec.org/a/gam/jsusta/v14y2022i19p12593-d932834.html
   My bibliography  Save this article

Effect of Thermophilic Microbial Agents on Antibiotic Resistance Genes and Microbial Communities during Co-Composting of Pig Manure and Tea Stalks

Author

Listed:
  • Xiaojia Chen

    (Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China
    School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China
    University of Chinese Academy of Sciences, Beijing 100049, China
    These authors equally contributed to this work.)

  • Chengjian Wu

    (Fuzhou Kaijie Foodstuff Development Co., Ltd., Fuzhou 350003, China
    These authors equally contributed to this work.)

  • Qinyu Li

    (Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China
    School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China
    University of Chinese Academy of Sciences, Beijing 100049, China)

  • Peng Zhou

    (School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China)

  • Zheng Chen

    (School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China)

  • Yang Han

    (School of Forestry, Northeast of Forestry University, Harbin 150040, China)

  • Jiping Shi

    (Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China
    School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China
    University of Chinese Academy of Sciences, Beijing 100049, China)

  • Zhijun Zhao

    (Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China
    University of Chinese Academy of Sciences, Beijing 100049, China)

Abstract

Antibiotic resistance caused by antibiotic resistance genes (ARGs) threatens human health. ARGs in animal manure can be degraded by composting. This study explored the changes in ARGs and microbial communities during co-composting of pig manure and tea stalks with (T) thermophilic microbial agents, including Geobacillus toebii ZF1 and Geobacillus sp. ZF2 for 15 days in a 4 L vacuum flask. Composting without thermophilic microbial agents served as control (CK). The results showed that the compost temperature of group T peaked at 72.1 °C and maintained above 70 °C for 4 days. The maximum temperature of group CK was 64.0 °C. The seed germination index showed that group T had reached maturity on day 9, while group CK reached maturity on day 15. The dominant bacteria in group CK were Bacillus . In group T, the dominant bacteria changed from Bacillus and Corynebacterium to Geobacillus . Compared with that of group CK, group T increased the removal of cmx , baeS and TaeA by 24.6%, 18.6% and 12.1%, respectively. Group T promoted and inhibited the removal of arlR and novA, respectively ( p < 0.05), while group CK showed the opposite effect. Network analysis suggested that group T effectively inactivated Dietzia , Clostridium and Corynebacterium ( p < 0.05) and promoted the removal of cmx and baeS . These results showed that thermophilic microbial agents could accelerate the maturation of pig manure and tea stalks, change microbial communities and promote the removal of ARGs. It is of great significance to reduce the spread of ARGs and, in turn, human health risks.

Suggested Citation

  • Xiaojia Chen & Chengjian Wu & Qinyu Li & Peng Zhou & Zheng Chen & Yang Han & Jiping Shi & Zhijun Zhao, 2022. "Effect of Thermophilic Microbial Agents on Antibiotic Resistance Genes and Microbial Communities during Co-Composting of Pig Manure and Tea Stalks," Sustainability, MDPI, vol. 14(19), pages 1-17, October.
    • Handle: RePEc:gam:jsusta:v:14:y:2022:i:19:p:12593-:d:932834
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2071-1050/14/19/12593/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/2071-1050/14/19/12593/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Li, Hongying & Yao, Chengli, 2017. "The influence of internal noise on the detection of hormonal signal with the existence of external noise in a cell system," Applied Mathematics and Computation, Elsevier, vol. 314(C), pages 1-6.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.

      Corrections

      All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jsusta:v:14:y:2022:i:19:p:12593-:d:932834. See general information about how to correct material in RePEc.

      If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

      If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

      If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

      For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

      Please note that corrections may take a couple of weeks to filter through the various RePEc services.

      IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.